Development of a method for metabolomic analysis of human exhaled breath condensate by gas chromatography-mass spectrometry in high resolution mode

Analytica Chimica Acta
A Peralbo-MolinaM D Luque de Castro

Abstract

Exhaled breath condensate (EBC) is a promising biofluid scarcely used in clinical analysis despite its non-invasive sampling. The main limitation in the analysis of EBC is the lack of standardized protocols to support validation studies. The aim of the present study was to develop an analytical method for analysis of human EBC by GC-TOF/MS in high resolution mode. Thus, sample preparation strategies as liquid-liquid extraction and solid-phase extraction were compared in terms of extraction coverage. Liquid-liquid extraction resulted to be the most suited sample preparation approach providing an average extraction efficiency of 77% for all compounds in a single extraction. Different normalization approaches were also compared to determine which strategy could be successfully used to obtain a normalized profile with the least variability among replicates of the same sample. Normalization to the total useful mass spectrometry signal (MSTUS) proved to be the most suited strategy for the analysis of EBC from healthy individuals (n = 50) reporting a within-day variability below 7% for the 51 identified compounds and a suited data distribution in terms of percentage of metabolites passing the Skewness and Kurtosis test for normality d...Continue Reading

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Citations

Jan 15, 2016·Journal of Chromatography. a·Yiman Wu, Liang Li
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ewelina P Dutkiewicz, Pawel L Urban
Oct 9, 2016·Biomedical Chromatography : BMC·Hajime MizunoToshimasa Toyo'oka
Jul 26, 2019·Journal of Breath Research·Alexander J SchmidtCristina E Davis
Mar 24, 2016·Journal of Breath Research·A Peralbo-MolinaM D Luque de Castro
Jul 27, 2018·Analytical and Bioanalytical Chemistry·Elaheh RahimpourAbolghasem Jouyban
May 5, 2017·Scientific Reports·A Peralbo-MolinaF Priego-Capote
Nov 26, 2020·Mass Spectrometry Reviews·Wei Han, Liang Li
Mar 12, 2021·Critical Reviews in Analytical Chemistry·Maryam KhoubnasabjafariAbolghasem Jouyban
Mar 16, 2021·Journal of Veterinary Pharmacology and Therapeutics·Sol M Rivera-VelezNicolas F Villarino

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