Development of a method for the quantification of D-glucaric acid in different varieties of grapefruits by high-performance liquid chromatography and mass spectra

Journal of Chromatography. a
Jose L PerezBhimanagouda S Patil


Grapefruits were found to contain D-glucaric acid, which has anticancer properties. In the present investigation, a method has been developed for the quantification of D-glucaric acid in grapefruit by high-performance liquid chromatography (HPLC) using a simple isocratic mobile phase with detection at 210 nm. Grapefruit samples were homogenized, centrifuged and filtered through a 0.45 microm membrane and injected into a HPLC system. The developed method was used for the quantification of D-glucaric acid in nine widely used grapefruit varieties. Furthermore, the identity of D-glucaric acid was confirmed by mass spectra. Seasonal variation of D-glucaric acid within the individual varieties were also measured in fruits harvested during November, February and May. The overall trend of D-glucaric acid level was increased from early to late season fruits. The developed method has a sensitivity of D-glucaric acid as low as 0.05 microg with an accuracy and precision >95%. This method was found to be simple, fast, accurate and reproducible. Moreover, the identity of D-glucaric acid was confirmed by mass spectra. Additionally, the labor intensity and cost of sample preparation were greatly reduced as compared to reported methods. This is...Continue Reading


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