Development of a microarray for studying porcine cytokine production in blood mononuclear cells and intestinal biopsies

Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
M AnderssonM Jensen-Waern


A microarray for demonstration of a limited number of porcine cytokines was initiated. Polymerase chain reaction (PCR) products were synthesized for four house-keeping genes, cyclophilin, beta-actin, hypoxanthine phosphoribosyltransferase (HPRT) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the following cytokines: interleukin (IL)-1beta, IL-4, IL-6, IL-8, IL-10, IL-12 p35, IL-12 p40, IL-18, interferon (IFN)-alpha, IFN-beta, IFN-gamma, tumour necrosis factor (TNF)-alpha, macrophage inhibition factor (MIF) and granulocyte/macrophage colony-stimulating factor (GM-CSF). Cytokine production was induced by incubation of porcine peripheral blood mononuclear cells (PBMC) with Concanavalin A (ConA) or oligodeoxynucleotide (ODN) 2216. RNA was isolated after 6 or 24 h from stimulated cells or unstimulated control cells and from intestinal biopsies. Cytokine expression was analysed using a 3-DNA Array 350(TM) labelling kit from Genisphere. Data were normalized using external control genes and analysed with the genepix pro 5.0 software. All the cytokines could be induced in PBMC and expressed on the array and the cytokines IL-6 and IFN-alpha were also analysed at protein level. All but one cytokine were expressed in samples fro...Continue Reading


Jun 1, 1992·Journal of Interferon Research·K ArturssonG Alm
Aug 29, 2000·Preventive Veterinary Medicine·H StegeS E Jorsal
Jun 8, 2002·Veterinary Immunology and Immunopathology·Elin JohanssonC Fossum
Jan 18, 2003·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Alexandre R CaetanoDaniel Pomp
Jan 25, 2003·The Veterinary Record·S ChouetS McOrist
Oct 11, 2003·Transplant Immunology·Paul J GibbsW Martin Howell
Oct 16, 2003·Physiological Genomics·William NobisPaul M Coussens
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Terence N LedgerIsabelle P Oswald
Jul 21, 2004·Veterinary Immunology and Immunopathology·Wenjing TaoByram Bridle
Sep 29, 2004·Journal of Biochemical and Molecular Toxicology·Supratim Choudhuri
Apr 6, 2005·Veterinary Immunology and Immunopathology·C M T DvorakM P Murtaugh
Jul 7, 2005·Journal of Muscle Research and Cell Motility·Marinus F W Te PasMarco H Pool
Sep 24, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Juliana G MachadoMichael P Murtaugh
Oct 15, 2005·Journal of Immunological Methods·J C DuvigneauM Gemeiner

❮ Previous
Next ❯


May 18, 2010·Veterinary Research Communications·M PietraA Peli
Jul 12, 2011·Veterinary Microbiology·M JacobsonM Jensen-Waern
Apr 13, 2010·Biochemical and Biophysical Research Communications·Hye-Ryeong JunMyung-Hee Kwon

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.