Development of a microbial high-throughput screening instrument based on elastic light scatter patterns.

The Review of Scientific Instruments
Euiwon BaeJ Paul Robinson

Abstract

A microbial high-throughput screening (HTS) system was developed that enabled high-speed combinatorial studies directly on bacterial colonies. The system consists of a forward scatterometer for elastic light scatter (ELS) detection, a plate transporter for sample handling, and a robotic incubator for automatic incubation. To minimize the ELS pattern-capturing time, a new calibration plate and correction algorithms were both designed, which dramatically reduced correction steps during acquisition of the circularly symmetric ELS patterns. Integration of three different control software programs was implemented, and the performance of the system was demonstrated with single-species detection for library generation and with time-resolved measurement for understanding ELS colony growth correlation, using Escherichia coli and Listeria. An in-house colony-tracking module enabled researchers to easily understand the time-dependent variation of the ELS from identical colony, which enabled further analysis in other biochemical experiments. The microbial HTS system provided an average scan time of 4.9 s per colony and the capability of automatically collecting more than 4000 ELS patterns within a 7-h time span.

References

Jan 1, 1994·Annual Review of Microbiology·B Swaminathan, P Feng
Jun 29, 1999·IEEE Transactions on Medical Imaging·K V AsariD Radhakrishnan
Jul 24, 2001·IEEE Transactions on Medical Imaging·J P HelfertyW E Higgins
Sep 12, 2001·Computer Methods and Programs in Biomedicine·J MarotzW Eisenbeiss
Aug 4, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jostein DahleOlav Kaalhus
Aug 17, 2004·Science·Nathalie Q BalabanStanislas Leibler
Jul 9, 2005·Journal of Immunological Methods·Michael PutmanMoon H Nahm
Jul 11, 2006·Journal of Biomedical Optics·Bulent BayraktarBartek Rajwa
Feb 17, 2007·Nature Reviews. Microbiology·Douglas B WeibelGeorge M Whitesides
Jun 15, 2007·IEEE Transactions on Pattern Analysis and Machine Intelligence·Richard Hartley, Sing Bing Kang
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Colin J InghamWillem M de Vos
Mar 5, 2008·Journal of Biomedical Optics·Euiwon BaeE Daniel Hirleman
May 3, 2008·Trends in Biotechnology·Orit Gefen, Nathalie Q Balaban
Oct 24, 2008·Biosensors & Bioelectronics·Padmapriya P BanadaArun K Bhunia
Sep 3, 2009·Journal of Food Protection·Byron Brehm-StecherMary Lou Tortorello
Feb 9, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matthew L ClarkeJeeseong Hwang
Jun 16, 2010·Journal of Biophotonics·Euiwon BaeE Daniel Hirleman
Nov 26, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bartek RajwaJ Paul Robinson
Jan 26, 2011·Journal of Microbiological Methods·Jiri GutJames H McKerrow
Feb 15, 2011·Journal of Microbiological Methods·Pier-Anne BélangerSébastien Roy

❮ Previous
Next ❯

Citations

Aug 2, 2013·The Review of Scientific Instruments·Joseph P SkinnerSergey Y Tetin
Nov 20, 2015·Expert Review of Anti-infective Therapy·Euiwon BaeJ Paul Robinson
Oct 25, 2016·Journal of Biomedical Optics·Huisung KimEuiwon Bae
Feb 11, 2014·Current Protocols in Cytometry·Dayong JinLawrence W Miller
May 15, 2013·Optics Express·Agnieszka SuchwalkoHalina Podbielska

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-imaging
feature extraction

Software Mentioned

Cytomat
Cytomat scheduler
LIBSVM
PixeLINK

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.