Development of a micromanipulation method for single cell isolation of prokaryotes and its application in food safety

PloS One
Marisa HohnadelRenaud Chollet

Abstract

For nearly a century, conventional microbiological methods have been standard practice for detecting and identifying pathogens in food. Nevertheless, the microbiological safety of food has improved and various rapid methods have been developed to overcome the limitations of conventional methods. Alternative methods are expected to detect low cell numbers, since the presence in food of even a single cell of a pathogenic organism may be infectious. With respect to low population levels, the performance of a detection method is assessed by producing serial dilutions of a pure bacterial suspension to inoculate representative food matrices with highly diluted bacterial cells (fewer than 10 CFU/ml). The accuracy of data obtained by multiple dilution techniques is not certain and does not exclude some colonies arising from clumps of cells. Micromanipulation techniques to capture and isolate single cells from environmental samples were introduced more than 40 years ago. The main limitation of the current micromanipulation technique is still the low recovery rate for the growth of a single cell in culture medium. In this study, we describe a new single cell isolation method and demonstrate that it can be used successfully to grow variou...Continue Reading

References

Nov 15, 2000·FEMS Microbiology Reviews·J Fröhlich, H König
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Jan 1, 2014·Journal of Microbiology and Biotechnology·Xihong ZhaoDeog Hwan Oh

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Citations

Apr 3, 2019·FEMS Microbiology Letters·Alejandra Hernandez-AgredaTracy D Ainsworth
May 16, 2020·Drug Delivery and Translational Research·Nilesh RaiVibhav Gautam
May 21, 2020·Micromachines·Adar HacohenOrit Shefi
Nov 19, 2019·Frontiers in Microbiology·Qin MaSenthil Subramanian
Aug 28, 2021·Foods·Nathanyelle Soraya Martins de AquinoEduardo Cesar Tondo

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Methods Mentioned

BETA
flow cytometry
Biopsy

Software Mentioned

R
CellTram

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