Development of a miniaturized stimulation device for electrical stimulation of cells

Journal of Biological Engineering
Gordon Minru XiongCleo Choong

Abstract

Directing cell behaviour using controllable, on-demand non-biochemical methods, such as electrical stimulation is an attractive area of research. While there exists much potential in exploring different modes of electrical stimulation and investigating a wider range of cellular phenomena that can arise from electrical stimulation, progress in this field has been slow. The reasons for this are that the stimulation techniques and customized setups utilized in past studies have not been standardized, and that current approaches to study such phenomena rely on low throughput platforms with restricted variability of waveform outputs. Here, we first demonstrated how a variety of cellular responses can be elicited using different modes of DC and square waveform stimulation. Intracellular calcium levels were found to be elevated in the neuroblast cell line SH-SY5Y during stimulation with 5 V square waves and, stimulation with 150 mV/mm DC fields and 1.5 mA DC current resulted in polarization of protein kinase Akt in keratinocytes and elongation of endothelial cells, respectively. Next, a miniaturized stimulation device was developed with an integrated cell chamber array to output multiple discrete stimulation channels. A frequency divi...Continue Reading

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Citations

May 8, 2018·Advanced Healthcare Materials·Jesus Paez-MayorgaAli Khademhosseini
Feb 23, 2020·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Liudmila LeppikJohn Howard Barker
Oct 10, 2020·Biomedical Microdevices·Chi-Sing Yuen, Hsiao-Chin Chen
Apr 10, 2020·Current Stem Cell Research & Therapy·Weidong ZhangFengxuan Han
Jan 6, 2021·BMC Biomedical Engineering·Christina N M RyanDimitrios I Zeugolis

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