Development of a Model for Chemotherapy-Induced Alopecia: Profiling of Histological Changes in Human Hair Follicles after Chemotherapy

The Journal of Investigative Dermatology
Ji-Seon YoonOh Sang Kwon

Abstract

Optimized research models are required to further understand the pathogenesis and prophylaxis of chemotherapy-induced alopecia. Our aim was to develop a mouse model for chemotherapy-induced alopecia by follicular unit transplantation of human hair follicles onto immunodeficient mice. Twenty-two weeks after transplantation, a single dose of cyclophosphamide (Cph) was administered to mice in the Cph100 (100 mg/kg) and Cph150 (150 mg/kg) groups. On day 6, hair follicles showed dystrophic changes, with swollen dermal papilla and ectopic melanin clumping in the hair bulb. In addition, upregulated expression of apoptotic regulators [P53, Fas/Fas-ligand, tumor necrosis factor-related apoptosis-inducing ligand/tumor necrosis factor-related apoptosis-inducing ligand receptor (TRAIL/TRAIL receptor), and Bax], increased apoptotic matrix keratinocytes, downregulated Ki67 expression, and decreased melanogenic protein in the hair bulb were noted in both groups. After 12 treatment days, hair follicles in Cph100 mice appeared to diminish dystrophic changes. In contrast, hair follicles of Cph150 mice prematurely entered a dystrophic catagen phase after 9 treatment days, and immunofluorescence staining for Ki67 and melanogenic protein expression...Continue Reading

Citations

Sep 13, 2019·EMBO Molecular Medicine·Talveen S PurbaRalf Paus
Dec 1, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jin Yong KimOhsang Kwon
Jul 20, 2020·The Journal of Investigative Dermatology·Iain S HaslamZhiCao Yue
Mar 20, 2021·Experimental Dermatology·Aimee FloresWilliam E Lowry
Nov 10, 2020·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Mi Hee KwackJae Hyun Park

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