Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

Theriogenology
Shuji TakabayashiHideki Katoh

Abstract

The common marmoset, Callithrix jacchus, is used as a New World monkey species in biomedical studies because of its small body size and good reproduction in captivity. A modified artificial insemination technique was developed in this species to encourage breeding of lines carrying interesting genes and traits. Fresh semen was collected by penile vibratory stimulation. Medium containing highly motile sperm was inseminated into the uterus using a catheter. Seven females were inseminated using freshly prepared sperm from different males every day for 3 days including the expected ovulation day. As a result, four females conceived, and three females delivered six offspring in total (two singletons and one quadruplet). The paternity of the newborns was determined using microsatellite markers to accurately pinpoint the timing of insemination and ovulation. It is expected that our artificial insemination protocol can be effectively used to establish marmoset lines and genetically manage marmoset colonies.

References

Oct 1, 1978·Journal of Child Psychology and Psychiatry, and Allied Disciplines·H Dienske, R Griffin
Mar 1, 1986·Journal of Reproduction and Fertility·J P Hearn
Jan 1, 1985·Journal of Reproduction and Fertility·P M SummersJ K Hodges
Oct 1, 1984·The Journal of Endocrinology·C R HarlowJ K Hodges
Sep 1, 1993·Journal of Reproduction and Fertility·M HeistermannJ K Hodges
Jan 1, 1997·American Journal of Primatology·J M MorrellJ K Hodges
Oct 17, 1998·Animal Reproduction Science·J M MorrellJ K Hodges
Jul 29, 2009·American Journal of Primatology·Hideki KatohToshio Itoh
Nov 26, 2009·Human Reproduction Update·Trevor G CooperKirsten M Vogelsong
Jul 28, 2011·Experimental Animals·Shuji Takabayashi, Hideki Katoh

❮ Previous
Next ❯

Citations

Oct 12, 2016·Scientific Reports·Jung Eun ParkAfonso C Silva
Jan 21, 2021·Journal of Medical Primatology·Jenna K SchmidtThaddeus G Golos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.