Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

Mycobiology
Fang-Jie YaoYoichi Honda

Abstract

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
May 21, 1998·Fungal Genetics and Biology : FG & B·P CallacJ M Olivier
Aug 8, 2012·Mycobiology·Jie Sun, Yinbing Bian
Feb 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I Paran, R W Michelmore

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Methods Mentioned

BETA
PCR

Software Mentioned

DNAMAN
Primer
BLASTN
BLAST

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