Development of a Molecular Tool for the Diagnosis of Leprosis, a Major Threat to Citrus Production in the Americas

Plant Disease
Eliane Cristina LocaliMarcos Antonio Machado

Abstract

Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The s...Continue Reading

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Citations

Jan 24, 2020·Journal of Economic Entomology·Ana Karen Beltran-BeltranPedro L Robles-García
Jun 9, 2016·Viruses·Pedro Luis Ramos-GonzálezJuliana Freitas-Astúa
Apr 19, 2017·Experimental & Applied Acarology·Aline Daniele TassiElliot W Kitajima
Feb 1, 2011·Experimental & Applied Acarology·K S KuboJ Freitas-Astúa
Dec 4, 2012·Experimental & Applied Acarology·Jose Carlos Verle Rodrigues, Carl C Childers
Mar 1, 2010·Plant Disease·Marinês BastianelJuliana Freitas-Astúa
Dec 13, 2018·Virusdisease·Marinês BastianelMaria A Nunes
Oct 24, 2008·Phytopathology·Marinês BastianelMarcos Antônio Machado
Apr 12, 2017·Phytopathology·Pedro Luis Ramos-GonzálezJuliana Freitas-Astúa
Jul 17, 2019·Journal of Economic Entomology·Renata Rodríguez-RamírezPedro L Robles-García
Dec 14, 2017·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Thierry Candresse
Aug 31, 2018·Frontiers in Plant Science·Gabriella D ArenaMarcos A Machado
Aug 5, 2019·Experimental & Applied Acarology·R Gómez-MercadoP L Robles-García
Sep 29, 2021·Experimental & Applied Acarology·Julio Domínguez-GabrielRebeca González-Gómez

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