Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: thiol strategy and detection using a sandwich ELISA

Toxicon : Official Journal of the International Society on Toxinology
Takeshi TsumurayaMasahiro Hirama

Abstract

Ciguatera fish poisoning (CFP) is a form of food poisoning caused by the ingestion of a variety of reef fish that have accumulated trace amounts of ciguatoxins produced by dinoflagellates of the genus Gambierdiscus through the food chain. CFP affects more than 50,000 people each year. The extremely low level of the causative neurotoxins, ciguatoxins, in fish has hampered the preparation of antibodies for detecting the toxins. In this paper, we describe a thiol strategy for synthesizing a keyhole limpet hemocyanin (KLH)-conjugate (20) of the ABCDE-ring fragment of the Pacific ciguatoxins, CTX1B (1) and 54-deoxyCTX1B (4). We succeeded in producing a monoclonal antibody (3G8) against the left wings of these ciguatoxins by immunizing mice with the hapten-KLH conjugate (20) as the synthetic antigen. The most promising mAb, 3G8, does not cross-react with other related marine toxins. Sandwich enzyme-linked immunosorbent assay (ELISA) utilizing 3G8 and the previously prepared monoclonal antibody (8H4) enabled us to detect 1 specifically at less than 0.28 ng/mL.

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Citations

Jan 7, 2014·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kentaro YogiTakeshi Yasumoto
Dec 23, 2014·Chemistry : a European Journal·Shuji YamashitaMasahiro Hirama
Dec 7, 2013·Molecular Nutrition & Food Research·Jonathan NicolasIvonne M C M Rietjens
Oct 12, 2016·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Masahiro Hirama
Feb 11, 2015·Expert Review of Clinical Immunology·Ahmad A Mourad, Sami L Bahna
Aug 6, 2020·Toxins·Tibor PasinszkiTodd E Dennis
Mar 6, 2021·The Journal of Organic Chemistry·Makoto SasakiKeisuke Arai
Apr 4, 2021·International Journal of Environmental Research and Public Health·Christopher R LoefflerDorina Bodi
Aug 10, 2020·Ecotoxicology and Environmental Safety·G GaianiM Campàs
May 18, 2018·Analytical Chemistry·Takeshi TsumurayaIkuo Fujii

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