Development of a Monoclonal Antibody-Based icELISA for the Detection of Ustiloxin B in Rice False Smut Balls and Rice Grains

Toxins
Xiaoxiang FuBaoming Wang

Abstract

Rice false smut is an emerging and economically-important rice disease caused by infection by the fungal pathogen Villosiclava virens. Ustiloxin B is an antimitotic cyclopeptide mycotoxin isolated from the rice false smut balls that formed in the pathogen-infected rice spikelets. A monoclonal antibody (mAb) designated as mAb 1B5A10 was generated with ustiloxin B-ovalbumin conjugate. A highly-sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. The median inhibitory concentration (IC50) of the icELISA was 18.0 ng/mL for the detection of ustiloxin B; the limit of detection was 0.6 ng/mL, and the calibration range was from 2.5 to 107.4 ng/mL. The LOD/LOQ values of the developed ELISA used for the determination of ustiloxin B in rice false smut balls and rice grains were 12/50 μg/g and 30/125 ng/g, respectively. The mAb 1B5A10 cross-reacted with ustiloxin A at 13.9% relative to ustiloxin B. Average recoveries of ustiloxin B ranged from 91.3% to 105.1% for rice false smut balls at spiking levels of 0.2 to 3.2 mg/g and from 92.6% to 103.5% for rice grains at spiking levels of 100 to 5000 ng/g. Comparison of ustiloxin B content in rice false smut balls and rice grains detected by ...Continue Reading

References

Apr 29, 1994·Biochemical Pharmacology·R F LudueńaS Iwasaki
Jun 19, 1998·The Journal of Antibiotics·N MorisakiS Iwasaki
Feb 15, 2001·Journal of Agricultural and Food Chemistry·M J MorenoA Montoya
Jul 17, 2001·Journal of Agricultural and Food Chemistry·P CliquetB M Goddeeris
May 2, 2006·Mycopathologia·Michael Z ZhengJohann Binder
Mar 6, 2009·The Journal of Veterinary Medical Science·Shigeru MiyazakiKazuhide Morimoto
Oct 31, 2012·International Journal of Molecular Sciences·Tijiang ShanLigang Zhou
Jul 16, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tijiang ShanLigang Zhou
Jun 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shiqiong LuLigang Zhou
Mar 18, 2015·Journal of Agricultural and Food Chemistry·Shiqiong LuLigang Zhou

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
enzyme-linked immunosorbent assays
ELISAs
ELISA
icELISA

Software Mentioned

OriginPro

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