Development of a monoclonal sandwich ELISA for the detection of animal and human Escherichia coli O157 strains

Journal of Applied Microbiology
P KerrH J Ball

Abstract

Production of a monoclonal antibody (MAb) to Escherichia coli O157 to develop a rapid test using a sandwich ELISA (sELISA) format. A MAb (7A6) was developed to the long-chain lipopolysaccharide of E. coli O157. A sELISA developed with the MAb reacted with 28 bovine and seven human enterohaemorrhagic E. coli (EHEC) O157 strains and also with two enterotoxigenic E. coli O157 strains. Cross-reaction to a rabbit diarrhoeal E.coli O15, Citrobacter freundii, Salmonella urbana and Vibrio cholerae O1 Inaba was detected. A MAb-based sELISA to detect E. coli O157 was produced. Its application to field samples is required to fully determine its prospective use for the detection of EHEC O157, to evaluate the non-specific interference of the cross-reacting strains. The assay produced is not wholly specific to EHEC O157, but has the potential to be used as a rapid method for screening large numbers of samples for E. coli O157.

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Citations

Jun 20, 2006·Immunology Letters·Hoon H SunwooJeong S Sim
Oct 6, 2005·Journal of Microbiological Methods·Burton W BlaisMartha Popela
Sep 8, 2016·PloS One·Xuehan ZhangKongwang He
Feb 14, 2003·Biotechnology and Bioengineering·Ramaiah Sachidanandham, Karina Yew-Hoong Gin
Aug 9, 2016·Journal of Food Protection·M FontcubertaM de Simón
Dec 11, 2014·Applied Biochemistry and Biotechnology·Dorra Ben Hamadou-CharfiDietrich Stephan
Feb 25, 2005·Journal of Food Protection·Burton W BlaisAmalia Martinez-Perez
Dec 18, 2017·Turkish Journal of Biology = Türk Biyoloji Dergisi·Dilek Çam, Hüseyin Avni Öktem
Dec 18, 2018·Chemical Reviews·Ariel L Furst, Matthew B Francis

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