Development of a Mouse Reporter Strain for the Purinergic P2X2 Receptor.

ENeuro
Seol-Hee KimThomas E Taylor-Clark

Abstract

The ATP-sensitive P2X2 ionotropic receptor plays a critical role in a number of signal processes including taste and hearing, carotid body detection of hypoxia, the exercise pressor reflex and sensory transduction of mechanical stimuli in the airways and bladder. Elucidation of the role of P2X2 has been hindered by the lack of selective tools. In particular, detection of P2X2 using established pharmacological and biochemical techniques yields dramatically different expression patterns, particularly in the peripheral and central nervous systems. Here, we have developed a knock-in P2X2-cre mouse, which we crossed with a cre-sensitive tdTomato reporter mouse to determine P2X2 expression. P2X2 was found in more than 80% of nodose vagal afferent neurons, but not in jugular vagal afferent neurons. Reporter expression correlated in vagal neurons with sensitivity to α,β methylene ATP (αβmATP). P2X2 was expressed in 75% of petrosal afferents, but only 12% and 4% of dorsal root ganglia (DRG) and trigeminal afferents, respectively. P2X2 expression was limited to very few cell types systemically. Together with the central terminals of P2X2-expressing afferents, reporter expression in the CNS was mainly found in brainstem neurons projecting...Continue Reading

Citations

Oct 31, 2020·British Journal of Pharmacology·Peter IllesFrancesco Di Virgilio
Apr 26, 2021·The International Journal of Biochemistry & Cell Biology·Aung Aung Kywe MoeStuart B Mazzone
May 30, 2021·Purinergic Signalling·Marcus GrohmannRalf Hausmann
Jul 2, 2021·Autonomic Neuroscience : Basic & Clinical·Alexander V Gourine, K Michael Spyer

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Methods Mentioned

BETA
PCR
genotyping
dissection
RNA-seq
transgenic

Software Mentioned

Excel
Fusion
NeuroTrace
Imaris
GraphPad Prism
Neurolucida
tdTomato

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