Development of a multibed sorption trap, comprehensive two-dimensional gas chromatography, and time-of-flight mass spectrometry system for the analysis of volatile organic compounds in human breath

Analytical Chemistry
Juan M Sanchez, Richard D Sacks

Abstract

A method for the determination of volatile organic compounds (VOCs) at sub-trace levels in breath samples based on a multibed sorption trap for the collection and concentration of VOCs, a comprehensive multidimensional gas chromatograph (GCxGC) for the separation of complex mixtures, and a time-of-flight mass spectrometer detector is designed and developed. The good performance of the trap tube device developed for the concentration together with the high sensitivity and separation power of the GCxGC results in a powerful system. In the analysis of samples, more than 100 different compounds are detected of which between 65 and 85 are clearly identified. A total of approximately 250 different compounds are observed in all the samples evaluated of which 142 are identified. A preliminary study to evaluate breath biomarkers for active smoking is performed. The levels of previously described biomarkers are found to be strongly time-dependent with amounts found approximately 1 h after smoking returning to the levels found in nonsmoking volunteers. However, 2,5-dimethylfuran, 2-methylfuran, and furan are found to be effective biomarkers given that they were only found in samples taken from smokers and could still be detected more than...Continue Reading

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Citations

Oct 22, 2009·Analytical and Bioanalytical Chemistry·Audrey N MartinMatthias Frank
Feb 27, 2010·Analytical and Bioanalytical Chemistry·Monica AlonsoJuan M Sanchez
Aug 1, 2014·Bioanalysis·Wolfram MiekischJochen K Schubert
Jan 22, 2015·Indoor Air·C J Weschler
Jun 15, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sabine KischkelJochen K Schubert
Oct 2, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J W DallingaF J van Schooten
Nov 12, 2014·Bioanalysis·Yong Foo WongPhilip J Marriott
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Jan 22, 2008·Journal of Chromatography. a·M AdahchourU A Th Brinkman
Apr 7, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mónica AlonsoJuan M Sanchez
Jul 17, 2018·Journal of Breath Research·Thomas Hector ChappuisBertrand Bourlon
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Oct 16, 2010·Advanced Powder Technology : the International Journal of the Society of Powder Technology, Japan·Alquam Mashir, Raed A Dweik

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