Development of a Multiplexed Assay for Detection of Leishmania donovani and Leishmania infantum Protein Biomarkers in Urine Samples of Patients with Visceral Leishmaniasis

Journal of Clinical Microbiology
Claudia AbeijonAntonio Campos-Neto

Abstract

Visceral leishmaniasis (VL) is a serious and fatal disease caused by the parasites Leishmania infantum and Leishmania donovani The gold standard diagnostic test for VL is the demonstration of parasites or their DNA in spleen, lymph node, or bone marrow aspirates. Serological tests exist but cannot distinguish active VL from either prior exposure to the parasites or previously treated VL disease. Using mass spectroscopy, we have previously identified three L. infantum protein biomarkers (Li-isd1, Li-txn1, and Li-ntf2) in the urine of VL patients and developed a sensitive and specific urine-based antigen detection assay for the diagnosis of VL that occurs in Brazil (where VL is caused by L. infantum). However, unpublished observations from our laboratory at DetectoGen showed that these biomarkers were detected in only 55% to 60% of VL patients from India and Kenya, where the disease is caused by L. donovani Here, we report the discovery and characterization of two new biomarkers of L. donovani (Ld-mao1 and Ld-ppi1) present in the urine of VL patients from these two countries. Capture enzyme-linked immunosorbent assays using specific rabbit IgG and chicken IgY were developed, and the assays had sensitivities of 44.4% and 28.8% for...Continue Reading

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Citations

Sep 20, 2019·PLoS Neglected Tropical Diseases·Angamuthu SelvapandiyanNirmal K Ganguly
Nov 1, 2020·Microorganisms·Antonio Campos-Neto, Claudia Abeijon
Oct 30, 2020·Frontiers in Cellular and Infection Microbiology·Yun WuGuowei Yang
Mar 12, 2021·Frontiers in Cellular and Infection Microbiology·Srija MoulikMitali Chatterjee
Dec 12, 2020·Talanta·Sultan AitekenovRostislav Bukasov
Jul 1, 2021·Future Medicinal Chemistry·Renan Augusto GomesGustavo Henrique Goulart Trossini

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