Development of a Neutralizing Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus S1 Protein

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Yunjing ZhangKegong Tian

Abstract

The truncated spike protein (S1, 390-789aa) of porcine epidemic diarrhea virus was expressed as a recombinant protein in Baculorvirus expression system. Female BALB/c mice were immunized with the purified recombinant S1 (rS1) protein and two monoclonal antibodies, designated 8A3A10 and 12C4G12, were generated by hybridoma technique. The two monoclonal antibodies specifically reacted with PEDV rS1 protein, as proven by Western blot and indirect immunofluorescence assay. 8A3A10 monoclonal antibody showed a neutralizing activity to PEDV, as demonstrated in the results of fluorescent focus neutralization assay. These two newly established monoclonal antibodies may work as a useful tool to study the pathogenic mechanisms of PED virus and to develop diagnostic reagents.

References

Oct 12, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Liyan CaoXiaofeng Ren
Feb 28, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ximei LeiChuanqing Wang

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis
fluorescence microscopy
Assay

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