Development of a new biomechanically defined single impact rabbit cartilage trauma model for in vivo-studies

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
Frank LeuchtRolf E Brenner

Abstract

Clinically oriented and easy to handle animal models are urgently needed to test pharmacologic treatment of cartilage trauma to reduce the resulting tissue damage by chondrocyte apoptosis and induction of matrix-degrading enzymes. To develop a biomechanically defined cartilage trauma model. We constructed a novel trauma device that allows biomechanically defined force application to the load-bearing region of the medial and lateral femoral condyles in adult rabbits. The fixation to the femur was specially designed to avoid uncontrolled influx of blood into the joint. The device was tested on the articular femoral surface of cadaveric rabbits. At a lower energy (1.0 J), the tests showed that superficial and partially deep fissuring, partial necrosis of the chondrocytes, and early proteoglycan loss occurred at the region of impact. Subchondral fractures could be excluded by micro CT. At higher energy (≥ 1.4 J), we observed more pronounced deep fissuring and in some cases complete shearing of the articular cartilage from the subchondral bone. Our model represents an easy to use method to create a biomechanically defined cartilage trauma and offers some advantages with respect to handling under aseptic surgical conditions and preve...Continue Reading

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Citations

Jun 19, 2014·International Journal of Molecular Medicine·Lei JinBaoan Ma
Sep 13, 2015·The American Journal of Sports Medicine·Helga JoosRolf E Brenner
Apr 13, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mieke NickienCorrinus C van Donkelaar
Oct 21, 2016·Journal of Biomechanical Engineering·Corinne R HenakLawrence J Bonassar
Jun 17, 2020·Journal of Biomechanical Engineering·Shannon K WalshCorinne R Henak
Jun 19, 2019·International Journal of Molecular Sciences·Jana RieggerRolf E Brenner

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