Development of a New Conditionally Immortalized Human Liver Sinusoidal Endothelial Cells

Biological & Pharmaceutical Bulletin
Meiyan ZhuTomomi Furihata

Abstract

Liver sinusoidal endothelial cells (LSECs), which are specialized endothelial cells that line liver sinusoids, have been reported to participate in a variety of liver functions, such as blood macromolecule clearance and factor VIII production. In addition, LSECs play crucial roles in liver regeneration following acute liver injury, as well as the development and progression of liver diseases or drug-induced hepatotoxicity. However, the molecular mechanisms underlying their roles remain mostly unknown. Therefore, in order to contribute to the clarification of those mechanisms, herein we report on the development of a new immortalized human LSEC (HLSEC) line. To produce this cell line, two immortalized genes were introduced into the primary HLSECs, which eventually resulted in the establishment of the HLSEC/conditionally immortalized, clone-J (HLSEC/ciJ). Consistent with the two-immortalized gene expression, HLSEC/ciJ showed excellent proliferation activity. Additionally, the results of gene expression analyses showed that several LSEC (as well as pan-endothelial) marker mRNAs and proteins were clearly expressed in HLSEC/ciJ. Furthermore, we found that adherence junction proteins were localized at the cell border in the HLSEC/ciJ...Continue Reading

References

Jul 3, 1999·The Journal of Biological Chemistry·H DoP Lollar
Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A McCourtS Johansson
May 29, 2004·Transplantation·Toshihisa MatsumuraNaoya Kobayashi
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sandra MarchSangeeta N Bhatia
May 3, 2013·The Journal of Clinical Investigation·Laurie D DeLeve
Jun 19, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ian A RoweJane A McKeating
Oct 2, 2015·Comprehensive Physiology·Karen Kristine SørensenBård Smedsrød

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