Development of a New Extraction Device Based on Parallel-Electromembrane Extraction

Analytical Chemistry
Nicolas DrouinJulie Schappler

Abstract

A new device for parallel-electromembrane extraction (Pa-EME) was developed to enable simultaneous and high-throughput extraction of ionic and ionizable compounds from biofluids. The new system is composed of a reusable conductive well-plate used as an acceptor compartment and a filtration well-plate used as a donor compartment. A design of experiments was implemented to optimize the main experimental parameters (agitation, voltage, and time) with standard solutions in formic acid 50 mM. The stirring rate was found the primary influent parameter. The Pa-EME device showed excellent extraction yields from 84% to 101% with RSD lower than 7.5% on model compounds. Optimized parameters were then applied to plasma samples and process efficiencies from 59% to 62% and RSD of less than 8.0% were obtained. The whole extraction process took less than 20 min to prepare 8 samples simultaneously, greatly enhancing the sample preparation throughput (<3 min per sample).

References

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Mar 18, 2016·Analytical Chemistry·Nicolas DrouinJulie Schappler

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Citations

Dec 20, 2018·Analytical and Bioanalytical Chemistry·Stig Pedersen-Bjergaard
Dec 8, 2017·The Analyst·Nicolas DrouinJulie Schappler
Jun 4, 2020·Journal of Separation Science·Magnus Saed RestanStig Pedersen-Bjergaard
Feb 4, 2021·Analytical Chemistry·Frøydis Sved SkottvollSteven Ray Wilson
Jul 7, 2018·Journal of Pharmaceutical and Biomedical Analysis·Nicolas DrouinJulie Schappler
Aug 13, 2021·Analytical Chemistry·Cong LiuChuixiu Huang
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li

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