Development of a new MLST scheme for differentiation of Fusarium solani Species Complex (FSSC) isolates

Journal of Microbiological Methods
Anne DebourgogneMarie Machouart

Abstract

Fungi belonging to the Fusarium solani Species Complex (FSSC) are well known plant pathogens. In addition to being the causative agent of some superficial infections, FSSC has recently emerged as a group of common opportunistic moulds, mainly in patients with haematological malignancies. Molecular typing methods are essential in order to better understand the epidemiology of such opportunistic agents with the final goal of preventing contamination. A three-locus typing scheme has thus been developed for FSSC; based on polymorphisms in the domains of the ITS, EF-1 alpha, and RPB2 genes. This method is now considered to be a useful reference for phylogenetic and taxonomic studies. In other significant clinical fungi (e.g., Candida sp., Cryptococcus neoformans, and Aspergillus fumigatus), genes coding for metabolic enzymes have been widely used and proven to be very informative for diagnosis and epidemiology. The contribution of these genes has never been evaluated for Fusarium sp. and more specifically for F. solani Species Complex. Here, we have evaluated the contribution of 25 genes for diagnosis and epidemiological purposes. We then report a new five-locus MLST scheme useful for diagnosis and typing of clinical FSSC isolates. ...Continue Reading

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Citations

Jun 15, 2013·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·A BernhardtK Tintelnot
Sep 4, 2015·International Journal of Medical Microbiology : IJMM·Anne BernhardtKathrin Tintelnot
Aug 25, 2015·Current Fungal Infection Reports·Anne D van DiepeningenCees Waalwijk
Jan 3, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Juan J LauthierPatricio Diosque
Feb 10, 2011·Virulence·Maged MuhammedEleftherios Mylonakis
Sep 13, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nicolás TomasiniPatricio Diosque
Aug 29, 2014·PLoS Neglected Tropical Diseases·Patricio DiosqueMatthew Yeo
Sep 25, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Xiaoli KeJianguo Wang
May 2, 2021·Journal de mycologie médicale·Priscila Dallé da RosaLuciano Zubaran Goldani

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