Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

Comparative Immunology, Microbiology and Infectious Diseases
Mustapha DahmaniOleg Mediannikov

Abstract

Bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. This pathology is at least partially caused by Anaplasma phagocytophilum, Anaplasma marginale, Anaplasma centrale, and Anaplasma bovis. The global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. In this study, we developed a new qPCR tool targeting the 23S rRNA gene for the detection of Anaplasmataceae bacteria. The primers and probe for the qPCR reaction had 100% specificity and could identify at least A. phagocytophilum, A. marginale, A. centrale, Anaplasma ovis, Anaplasma platys, Ehrlichia canis, Ehrlichia ruminantium, Neorickettisa sennetsu, and Neorickettsia risticii. We used this tool to test samples of bovines from Batna (Algeria), an area from which bovine anaplasmosis have never been reported. We identified three genetic variants of A. phagocytophilum, A. platys and Anaplasma sp. "variant 4". This finding should attract the attention of public authorities to assess the involvement of these pathogens in human and animal health.

References

Mar 1, 1991·The Journal of Infectious Diseases·J E DawsonD B Fishbein
Jul 1, 1994·International Journal of Systematic Bacteriology·A M VialeR G Ferreyra
Jan 15, 2003·Clinical Microbiology Reviews·Christopher D Paddock, James E Childs
Apr 1, 2004·Journal of Comparative Pathology·L Bell-Sakyi
Oct 16, 2004·Veterinary Parasitology·Enara AguirreMiguel A Tesouro
Jul 22, 2005·Microbiology and Immunology·Hyo-Soon ParkSeung-Hyun Lee
Jul 27, 2005·Veterinary Parasitology·José de la FuenteKatherine M Kocan
Nov 23, 2006·Annals of the New York Academy of Sciences·Yasuko Rikihisa
Nov 23, 2006·Annals of the New York Academy of Sciences·Zerai Woldehiwet
Oct 9, 2009·Veterinary Parasitology·Katherine M KocanS A Ewing
Jun 2, 2010·Clinics in Laboratory Medicine·Nahed IsmailJere W McBride
Nov 3, 2010·Transboundary and Emerging Diseases·P Aubry, D W Geale
May 29, 2012·Infectious Disease Clinics of North America·Tahar KernifPhilippe Parola
Nov 28, 2012·Ticks and Tick-borne Diseases·Khamsa MokraniPierre-Edouard Fournier
Apr 5, 2013·Tropical Animal Health and Production·Mamadou Lamine DjibaMady Ndiaye
Nov 6, 2013·The Journal of Veterinary Medical Science·Adrian Patalinghug YbañezHisashi Inokuma
Dec 7, 2013·Tropical Animal Health and Production·Jenevaldo Barbosa da Silva, Adivaldo Henrique da Fonseca
Mar 5, 2014·Transboundary and Emerging Diseases·E ZweygarthL M F Passos
Oct 1, 2014·The American Journal of Tropical Medicine and Hygiene·Cruz M Arraga-AlvaradoEdward B Breitschwerdt
Jan 15, 2015·Ticks and Tick-borne Diseases·Mustapha DahmaniBernard Davoust
Feb 2, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Naouelle AzzagHenri-Jean Boulouis

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Citations

May 3, 2016·Ticks and Tick-borne Diseases·Claudia Bezerra da SilvaCarlos Luiz Massard
Sep 27, 2016·Ticks and Tick-borne Diseases·Mustapha DahmaniOleg Mediannikov
Jan 26, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mara BattilaniFrancesco Dondi
Jan 29, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mourad Ben SaidLilia Messadi
Apr 4, 2017·Acta Tropica·C B EhounoudO Mediannikov
Jul 18, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Claudia B da SilvaCarlos L Massard
Mar 23, 2018·Transboundary and Emerging Diseases·G V GioiaG Zanella
Apr 18, 2018·PLoS Neglected Tropical Diseases·Meriem LouniOleg Mediannikov
Jul 18, 2020·Vector Borne and Zoonotic Diseases·Wei WeiYan-Ling Hu
Dec 19, 2018·Parasitology Research·Rangapura Kariyappa PradeepReghu Ravindran
Jan 16, 2016·PLoS Neglected Tropical Diseases·Cyrille Bilé EhounoudOleg Mediannikov
Oct 22, 2016·Parasites & Vectors·Youmna M'ghirbiAli Bouattour
Oct 30, 2020·Pathogens·Ghania BoulariasHenri-Jean Boulouis
Apr 2, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Vera RarNina Tikunova
Jul 14, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Mehdi BoucheikhchoukhPhilippe Parola
Aug 29, 2021·Pathogens·Kimberly J LedgerSamantha M Wisely
Sep 30, 2021·PLoS Neglected Tropical Diseases·Hend H A M AbdullahOleg Mediannikov

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