Development of a new protocol for rapid bacterial identification and susceptibility testing directly from urine samples

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Yuliya ZboromyrskaJordi Vila

Abstract

The current gold standard method for the diagnosis of urinary tract infections (UTI) is urine culture that requires 18-48 h for the identification of the causative microorganisms and an additional 24 h until the results of antimicrobial susceptibility testing (AST) are available. The aim of this study was to shorten the time of urine sample processing by a combination of flow cytometry for screening and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for bacterial identification followed by AST directly from urine. The study was divided into two parts. During the first part, 675 urine samples were processed by a flow cytometry device and a cut-off value of bacterial count was determined to select samples for direct identification by MALDI-TOF-MS at ≥5 × 10(6) bacteria/mL. During the second part, 163 of 1029 processed samples reached the cut-off value. The sample preparation protocol for direct identification included two centrifugation and two washing steps. Direct AST was performed by the disc diffusion method if a reliable direct identification was obtained. Direct MALDI-TOF-MS identification was performed in 140 urine samples; 125 of the samples were positive by urine culture, 12 w...Continue Reading

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Citations

Oct 30, 2016·Expert Review of Molecular Diagnostics·Teresa CimminoJean-Marc Rolain
Nov 5, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B ChenS Liang
Mar 2, 2017·Nature Reviews. Urology·Michael DavenportJoseph C Liao
Jan 22, 2017·Enfermedades infecciosas y microbiología clínica·María Siller-RuizJuan Luis Muñoz-Bellido
Jun 24, 2017·Chemical Society Reviews·Linda VáradiPaul W Groundwater
Nov 6, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Claire Périllaud-DuboisJean-Claude Nguyen Van
Sep 15, 2018·Annals of Laboratory Medicine·Matthijs Oyaert, Joris Delanghe
Sep 27, 2018·Frontiers in Genetics·Pria N GhoshKieran A Bates
Jan 26, 2017·The Journal of Antimicrobial Chemotherapy·Marina OviañoGermán Bou
Apr 25, 2021·Diagnostic Microbiology and Infectious Disease·Stefan RiedelG Terrance Walker

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