Development of a novel assay to evaluate the functional potential of umbilical cord blood progenitors

Transfusion
Jo-Anna ReemsIvan N Rich

Abstract

Although the colony-forming cell (CFC) assay provides the most relevant information regarding the functional potential of progenitors in a unit of umbilical cord blood (UCB), technical challenges associated with this assay have made it difficult to standardize the assay among testing laboratories. The purpose of this study was to assess the reproducibility of a newly developed functional assay (HALO SPC-QC [HALO], HemoGenix, Inc.). This test is based on the principle that cellular proliferation responses to cytokine stimuli are proportional to intracellular ATP levels from progeny cells generated in culture from progenitors. Results of the HALO assay were evaluated at two geographically distinct sites with matched aliquots from 12 different UCB units. A significant correlation between the two sites for total nucleated cell counts was observed (r = 0.98, p < 0.001). Similarly, a strong correlation between HALO results from both sites was observed (r = 0.94, p < 0.001). Also, despite using different methods at each site for the CFC assay, results from the two sites correlated (r = 0.79, p = 0.002). A good correlation between the CFC and HALO assays (r = 0.73, p < 0.005), however, was only observed at the site with the same cytoki...Continue Reading

References

Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Aug 1, 1970·Journal of Cellular Physiology·B L Pike, W A Robinson
Oct 1, 1966·Experimental Cell Research·D H Pluznik, L Sachs
Dec 1, 1982·British Journal of Haematology·I N Rich, B Kubanek
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·P RubinsteinC E Stevens
Mar 15, 1993·Journal of Immunological Methods·S P CrouchJ Fletcher
Dec 1, 1996·Journal of Hematotherapy·I D LewisL B To
Nov 26, 1998·The New England Journal of Medicine·P RubinsteinC E Stevens
Jan 19, 2002·Journal of Hematotherapy & Stem Cell Research·A M de KreukA R Jonkhoff
Nov 5, 2003·Journal of Hematotherapy & Stem Cell Research·Irène DoboSylvie Hermouet
Nov 27, 2004·The New England Journal of Medicine·Vanderson RochaUNKNOWN Eurocord-Netcord Registry
Jul 9, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ivan N Rich, Karen M Hall
Jan 31, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Paul L MartinJoanne Kurtzberg
Apr 6, 2006·Transfusion·Gary MoroffUNKNOWN Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Apr 13, 2007·Expert Opinion on Drug Metabolism & Toxicology·Ivan N Rich

❮ Previous
Next ❯

Citations

Jan 21, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yun SunXiao-Dong Chen
Apr 9, 2013·Journal of Tissue Engineering and Regenerative Medicine·Pedro Z AndradeCláudia L da Silva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.