Development of a novel beta-cell specific promoter system for the identification of insulin-producing cells in in vitro cell cultures

Experimental Cell Research
Takuya FukazawaNoriaki Tanaka

Abstract

Recently, it has been reported that islet transplantation into patients with Type 1 diabetes may achieve insulin independence for a year or longer [Shapiro et al., Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen, N Engl J Med. 343 (2000) 230-238]. However, the amount of donor islet tissue is limited, therefore, multiple approaches are being explored to generate insulin-producing cells in vitro. Some promising results have been obtained using mouse and human stem cells and progenitor cells [Soria et al., From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus, Diabetologia. 4 (2001) 407-415; Lechner et al., Stem/progenitor cells derived from adult tissues: potential for the treatment of diabetes mellitus, Am J Physiol Endocrinol Metab. 284 (2003) 259-266; Bonner-Weir et al., In vitro cultivation of human islets from expanded ductal tissue, Proc Natl Acad Sci U S A, 97 (2000) 7999-8004; Assady et al., Insulin production by human embryonic stem cells, 50 (2001) Diabetes 1691-1697]. However, the efficiency of obtaining populations with high numbers of differentiated cells has been poor. In order to improve the efficiency of produc...Continue Reading

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Citations

Jan 21, 2014·Seminars in Cell & Developmental Biology·Maik DahlhoffMarlon R Schneider
Apr 2, 2009·Current Opinion in Organ Transplantation·Carmella Evans-MolinaRaghavendra G Mirmira
Mar 28, 2009·Biochemical and Biophysical Research Communications·Bing XiaBaofeng Yang
Oct 28, 2020·Journal of Cellular Physiology·Selda Gezginci-OktayogluSehnaz Bolkent
Mar 18, 2022·Human Gene Therapy·Sunghee ChaiMarkus Grompe

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