Development of a novel chemokine-mediated in vivo T cell recruitment assay.

Journal of Immunological Methods
Gabriele S V CampanellaAndrew D Luster

Abstract

Trafficking of leukocytes to sites of inflammation is an important step in the establishment of an immune response. Chemokines are critical regulators of leukocyte trafficking and are widely studied molecules for their important role in disease and for their potential as new therapeutic targets. The ability of chemokines to induce leukocyte recruitment has been mainly measured by in vitro chemotaxis assays, which lack many components of the complex biological process of leukocyte migration and therefore provide incomplete information about chemokine function in vivo. In vivo assays to study the activity of chemokines to induce leukocyte recruitment have been difficult to establish. We describe here the development of a robust in vivo recruitment assay for CD8(+) and CD4(+) T lymphocytes induced by the CXCR3 ligands IP-10 (CXCL10) and I-TAC (CXCL11). For this assay, in vitro activated T lymphocytes were adoptively transferred into the peritoneum of naïve mice. Homing of these transferred T lymphocytes into the airways was measured following intratracheal instillation of chemokines. High recruitment indices were achieved that were dependent on chemokine concentration and CXCR3 expression on the transferred lymphocytes. Recruitmen...Continue Reading

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Citations

Jan 12, 2011·Immunology and Cell Biology·Joanna R Groom, Andrew D Luster
Mar 8, 2011·Experimental Cell Research·Joanna R Groom, Andrew D Luster
Jul 30, 2010·PloS One·Sandhya Thulasi DasKrishna Rajarathnam
Sep 22, 2010·PloS One·Gabriele S V CampanellaAndrew D Luster
Apr 20, 2013·Nature Reviews. Immunology·David Masopust, Jason M Schenkel
May 11, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edward SeungAndrew D Luster
Apr 27, 2018·Physiological Reports·Michael R DeyhleRobert D Hyldahl
Oct 16, 2018·Frontiers in Medicine·Paula T KuoGraham R Leggatt
Mar 25, 2020·Cellular Oncology (Dordrecht)·Hossein BagheriHamed Mirzaei
May 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Courtney L ErskineKeith L Knutson
Jan 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steve OghumuAbhay R Satoskar
Feb 26, 2021·Nature Reviews. Immunology·Deborah J Fowell, Minsoo Kim
Dec 6, 2017·Cancer Treatment Reviews·Ryuma TokunagaHeinz-Josef Lenz
May 21, 2021·Frontiers in Immunology·Jingqiao ChenHuasheng Yang

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