Development of a novel flow cytometric immunobead array to quantify VWF: Ag and VWF: GPIbR and its application in acute myocardial infarction

European Journal of Haematology
Bin YanYiming Zhao

Abstract

Both von Willebrand disease (VWD) and acute myocardial infarction (AMI) involve quantitative and qualitative changes in von Willebrand factor (VWF). Our objective was to develop a rapid and precise flow cytometric immunobead array (FCIA) to quantify VWF antigen (VWF:Ag) and ristocetin-triggered platelet glycoprotein Ib binding (VWF:GPIbR) and apply it in a clinical setting. Microbeads, coated with monoclonal antibodies for SZ29 or SZ151 IgG, were incubated with diluted plasma. VWF-binding microbeads were detected with FITC-conjugated sheep-anti-human VWF IgG by flow cytometry. Plasma VWF:Ag and VWF:GPIbR levels in normal controls (CTL; n=105), patients with VWD (n=21), and patients with AMI (n=146) were tested by FCIA and ELISA in parallel. ADAMTS13 activity and VWF multimer analyses were also implemented. Our novel FCIA showed a strong correlation with the ELISA results (VWF:Ag, r=.855; VWF:GPIbR, r=.813). The intra-assay coefficient variations (CVs) of VWF:Ag-FCIA and VWF:GPIbR-FCIA were 9.2% and 7.7%, respectively, and the interassay CVs were 12.6% and 13.5%, respectively. Plasma VWF:Ag and VWF:GPIbR levels were significantly higher in patients with AMI than in CTL (P<.0001), whereas the ratios of ADAMTS13/VWF:Ag and ADAMTS1...Continue Reading

References

Sep 1, 1985·The Journal of Clinical Investigation·L A SpornD D Wagner
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E A JaffeR L Nachman
Mar 17, 1999·American Journal of Clinical Pathology·A C KempferM A Lazzari
Feb 28, 2001·The New England Journal of Medicine·T C WascherI Schmoelzer
Jun 24, 2003·Scandinavian Journal of Clinical and Laboratory Investigation·T L LindahlA Larsson
Aug 11, 2006·Journal of Thrombosis and Haemostasis : JTH·K KaikitaH Ogawa
Oct 21, 2006·Blood·Chan K N K ChionFrits R Rosendaal
Aug 28, 2007·The American Journal of Cardiology·Masakazu MatsukawaHisao Ogawa
Aug 29, 2007·Journal of Thrombosis and Haemostasis : JTH·Y ZhaoC Ruan
Mar 19, 2008·Circulation·Alexander O SpielBernd Jilma
Jan 5, 2011·Thrombosis Research·Emmanuel J Favaloro
Sep 3, 2011·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Emmanuel J Favaloro
Apr 11, 2015·Journal of Thrombosis and Haemostasis : JTH·I BodóUNKNOWN von Willebrand factor Subcommittee of the Standardization and Scientific Committee of the International Society for Thrombos
Apr 29, 2015·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Le QuYiming Zhao
Dec 30, 2015·Journal of Thrombosis and Haemostasis : JTH·A de Jong, J Eikenboom
Sep 16, 2016·Advances in Experimental Medicine and Biology·Minoo Shahidi

❮ Previous
Next ❯

Citations

Jan 23, 2020·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Bin YanYiming Zhao
Oct 13, 2018·European Cardiology·Ricardo A Perez de la HozAlejandra Silvia Scazziota

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

© 2022 Meta ULC. All rights reserved