Development of a novel fluorometric assay for nitric oxide utilizing sesamol and its application to analysis of nitric oxide-releasing drugs

Luminescence : the Journal of Biological and Chemical Luminescence
Seiji AbeH Arakawa

Abstract

Nitric oxide (NO) is related to various physiological effects as well as to numerous diseases caused by accentuation of NO production. Measurement of NO in cells and tissues is difficult as NO readily reacts with other molecules; furthermore, its half-life as a radical is fleeting. Currently, many NO pharmaceuticals are marketed as therapeutic agents for ischemic disease. Consequently, the identification of NO radicals and determination of generation rate from pharmaceuticals is very important when the effect of the medicinal supply is estimated. In this study, we developed a fluorometric assay for NO employing sesamol (3,4-methylenedioxyphenol) as a fluorometric substrate. Sesamol is converted to a fluorescent derivative (ex. 365 nm, em. 447 nm), which is dimmer in the presence of NO. The detection limit of NO with this method is 400 fmol; moreover, NO generated from drugs can be measured.

References

Jan 1, 1988·Ultrasound in Medicine & Biology·J R McLean, A J Mortimer
Jan 1, 1988·Free Radical Biology & Medicine·R A Kloner
Jul 1, 1968·Analytical Chemistry·G G GuilbaultM Juneau
Aug 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S P Mahadik, R E Scheffer
Mar 21, 1998·Chemical & Pharmaceutical Bulletin·H KojimaT Nagano

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Citations

Oct 27, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ken-Ichi OhnoTsutomu Fujimura

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