Development of a novel hybrid bioactive hydrogel for future clinical applications

Journal of Biomaterials Applications
Lydia FrancisTahera Ansari

Abstract

Three-dimensional hydrogels are ideal for tissue engineering applications due to their structural integrity and similarity to native soft tissues; however, they can lack mechanical stability. Our objective was to develop a bioactive and mechanically stable hydrogel for clinical application. Auricular cartilage was decellularised using a combination of hypertonic and hypotonic solutions with and without enzymes to produce acellular tissue. Methacryloyl groups were crosslinked with alginate and PVA main chains via 2-aminoethylmathacrylate and the entire macromonomer further crosslinked with the acellular tissue. The resultant hydrogels were characterised for its physicochemical properties (using NMR), in vitro degradation (via GPC analysis), mechanical stability (compression tests) and in vitro biocompatibility (co-culture with bone marrow-derived mesenchymal stem cells). Following decellularisation, the cartilage tissue showed to be acellular at a significant level (DNA content 25.33 ng/mg vs. 351.46 ng/mg control tissue), with good structural and molecular integrity of the retained extra cellular matrix (s-GAG= 0.19 μg/mg vs. 0.65 μg/mg ±0.001 control tissue). Proteomic analysis showed that collagen subtypes and proteoglycans w...Continue Reading

References

Jun 1, 1990·Journal of Biomedical Materials Research·T KokuboT Yamamuro
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Feb 20, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·A Werner, W Gründer
Nov 15, 2000·Journal of Biomedical Materials Research·K A SmedsM W Grinstaff
Aug 24, 2005·Biomaterials·Abhijeet JoshiMichele Marcolongo
Jun 8, 2006·Cell Biochemistry and Biophysics·Melvin SchindlerSally Meiners
Jul 7, 2007·Methods in Cell Biology·Kandice R JohnsonValerie M Weaver
Sep 22, 2007·Biomacromolecules·Martin EhrbarMatthias P Lutolf
Oct 22, 2008·Acta Biomaterialia·Stephen F BadylakThomas W Gilbert
Jan 1, 2009·Tissue Engineering. Part C, Methods·Shiri UrielEric M Brey
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Feb 13, 2010·Biomaterials·Seiichi FunamotoAkio Kishida
Jun 17, 2010·Molecular & Cellular Proteomics : MCP·Athanasios DidangelosManuel Mayr
Aug 7, 2010·International Journal of Biomaterials·John C FitzpatrickFranco M Capaldi
Dec 3, 2010·Journal of Cell Science·Christian FrantzValerie M Weaver
Dec 18, 2010·The International Journal of Biochemistry & Cell Biology·Andreas R KlattRaimund Wagener
Feb 8, 2011·Biomaterials·Peter M CrapoStephen F Badylak
Sep 8, 2012·Nature·Jeong-Yun SunZhigang Suo
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sidi A BencherifDavid J Mooney
Nov 12, 2014·Journal of Laboratory Automation·Ki-Hwan NamDeok-Ho Kim

❮ Previous
Next ❯

Citations

Oct 11, 2019·Journal of Materials Science. Materials in Medicine·Marta Calvo CatoiraFrancesca Boccafoschi
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Claudia BilodeauMartin Post
Oct 24, 2020·Biomedicines·Maria Vittoria GiraudoFrancesca Boccafoschi

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
electrophoresis
nuclear magnetic resonance
NMR
dissection
FCS
Scanning electron microscopy

Software Mentioned

ProteoWizard
Mascot
Bluehill
Central Proteome Facility Pipeline ( CPFP )
OMSSA
Jeol
MS Concert
iProphet
Prism
! Tandem

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.