Development of a novel methodology for in vivo quantification of N/O/S-containing polycyclic aromatic hydrocarbons located on the epidermis of mangrove roots using graphene quantum dots as a fluorescence quencher

Marine Pollution Bulletin
Ruilong LiKefu Yu

Abstract

A novel approach for in vivo determination of typical N/O/S-containing PAHs located on the epidermis of mangrove roots was developed using graphene quantum dots (GQDs) as a fluorescence quencher. The decreasing fluorescence intensity from GQDs was attributed to the amount of N/O/S-containing PAHs introduced onto the epidermis of mangrove roots. The linear ranges of the proposed method were 10.3-980ngg-1, 9.5-1350ngg-1 and 7.8-1200ngg-1 for DBF, DBT and CAR located on the epidermis of K. obovata roots, respectively. This method was also shown to be valid for quantifying the N/O/S-containing PAHs on the root epidermis in the presence of heavy metal (10mmolL-1) and dissolved organic matter (1mgL-1 C). Moreover, the death rates of epidermal cells were almost unchanged (p>0.05) after acquiring the fluorescence spectra, which is superior to the previously reported LITRF method with which the cell death rates increased to 42.6%.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.