Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine

Theranostics
Do Thi Hoang KimSeon-Ju Yeo

Abstract

Zika virus (ZIKV) has been identified as a cause of adverse outcomes of pregnancy, including microcephaly and other congenital diseases. Most people infected with ZIKV do not show any symptoms. Development of a method to discriminate dengue virus (DENV) and ZIKV infections has been challenging, and efficient assays for patient management are limited, attributable to high levels of cross-reactivity among co-circulating Flaviviruses. Thus, there is an urgent need for a specific high-throughput diagnostic assay to discriminate ZIKV infections from other Flavivirus infections. Methods: A novel epitope peptide of the ZIKV envelope protein was predicted using three immune epitope database analysis tools and then further modified. A molecular docking study was conducted using three-dimensional structures of the ZIKV envelope and peptide. Experimentally, interactions between the selected peptides and virus were assessed via a fluorescence-linked sandwich immunosorbent assay (FLISA), and performance of peptide-linked sandwich FLISA was evaluated in virus-spiked human serum and urine. Results: The Z_10.8 peptide (KRAVVSCAEA) was predicted to be a suitable detector, with a higher binding affinity than other candidates based on four criter...Continue Reading

Citations

Jul 18, 2019·International Journal of Molecular Sciences·Ngoc Minh NguyenSeon-Ju Yeo
Dec 31, 2020·International Journal of Molecular Sciences·Ana Paula Corrêa ArgondizzoSotiris Missailidis
May 1, 2021·International Journal of Molecular Sciences·Tae-Hyeong Kim, Seong-Wook Lee
Jul 3, 2021·Pharmaceuticals·Arne KrügerCarsten Wrenger
Jul 11, 2021·Biosensors & Bioelectronics·Marcello MasciniSylvia Daunert
Jul 10, 2021·World Journal of Microbiology & Biotechnology·Tholasi Nadhan NavienMarimuthu Citartan

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Datasets Mentioned

BETA
AHG97600.1

Methods Mentioned

BETA
fluorescent-linked immunosorbent assay
Fluorescence
reverse transcription PCR
in vitro transcription
PCR
enzyme-linked immunoassay
ELISAs
ELISA

Software Mentioned

BCPreds
tasser
AutoDock Vina
ABCpred
PyMOL
PyRx
Autodok Vina
Primer Express
Bepipred
GraphPad Prism

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