Development of a novel proteomic approach for mitochondrial proteomics from cardiac tissue from patients with atrial fibrillation.

Journal of Proteome Research
Maryam GoudarziNiv Ad

Abstract

Atrial fibrillation (AF) is the most common cardiac arrhythmia affecting approximately 2.2 million Americans. Because several studies have suggested that changes in mitochondrial function and morphology may contribute to AF, we developed a novel proteomic workflow focused on the identification of differentially expressed mitochondrial proteins in AF patients. Right human atrial tissue was collected from 20 patients, 10 with and 10 without AF, and the tissue was subjected to hydrostatic pressure cycling-based lysis followed by label-free mass spectrometric (MS) analysis of mitochondrial enriched isolates. Approximately 5% of the 700 proteins identified by MS analysis were differentially expressed between the AF and non-AF samples. We chose four differentially abundant proteins for further verification using reverse phase protein microarray analysis based on their known importance in energy production and regulatory association with atrial ion channels: four and a half LIM, destrin, heat shock protein 2, and chaperonin-containing TCP1. These initial study results provide evidence that a workflow to identify AF-related proteins that combines a powerful upfront tissue cell lysis with high resolution MS for discovery and protein arr...Continue Reading

References

Jan 1, 1982·Circulation·W T Friedewald, C D Furberg
Feb 24, 2001·Journal of the American College of Cardiology·S S ChughB J Gersh
Nov 14, 2001·Circulation·V KühlkampC Mewis
Dec 12, 2001·Journal of Molecular and Cellular Cardiology·J AusmaM Borgers
Jan 24, 2002·Nature·Stanley Nattel
Jun 14, 2002·Cardiovascular Research·Maurits AllessieUlrich Schotten
Nov 15, 2002·Journal of Cell Science·Stephan LangeElisabeth Ehler
Mar 29, 2005·Molecular and Cellular Biochemistry·Evelin SeppetEnn K Seppet
Oct 5, 2006·Journal of Interventional Cardiac Electrophysiology : an International Journal of Arrhythmias and Pacing·Aamir CheemaHugh Calkins
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Zhanna MakazanNaranjan S Dhalla
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Valerie S CalvertZobair M Younossi
Sep 25, 2007·The Annals of Thoracic Surgery·Basel RamlawiFrank W Sellke
Feb 2, 2008·Journal of the American College of Cardiology·Manuel MayrA John Camm
Mar 11, 2008·Cardiovascular Research·Bianca J J M BrundelHarm H Kampinga
Feb 4, 2010·Expert Review of Proteomics·Deborah H LundgrenDavid K Han

❮ Previous
Next ❯

Citations

Apr 29, 2014·BioMed Research International·Ashling Holland, Kay Ohlendieck
Jul 28, 2015·The Annals of Thoracic Surgery·Niv AdGraciela Pritchard
Jun 13, 2014·Proteomics. Clinical Applications·Xiaomeng ShenJun Qu
Nov 17, 2012·Journal of Proteomics·Parveen SharmaAnthony O Gramolini
Apr 14, 2015·Journal of Proteomics·Peter W LindingerJohn E Walker
Oct 4, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Niv Ad
Nov 15, 2017·Nature Communications·Sophia DollMatthias Mann
May 14, 2018·Proteomics. Clinical Applications·Oliver KleinSalah A Mohamed
Oct 18, 2012·Circulation. Arrhythmia and Electrophysiology·Ayesha I De Souza, A John Camm
Jan 20, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Yvonne DöringChristian Weber
Dec 19, 2018·Circulation. Genomic and Precision Medicine·Caroline J CoatsPerry M Elliott
Dec 21, 2016·Expert Review of Proteomics·Marika MokouHarald Mischak
Jan 18, 2021·Pflügers Archiv : European journal of physiology·Julie H RennisonDavid R Van Wagoner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.