PMID: 2111868Jan 1, 1990Paper

Development of a novel scintillation proximity radioimmunoassay for platelet-activating factor measurement: comparison with bioassay and GC/MS techniques

Life Sciences
J SugataniK Saito

Abstract

A novel, facile and sensitive scintillation proximity radioimmunoassay (SPRIA) for quantitation of PAF has been developed. No separation of antibody bound [3H]PAF from free [3H]PAF is required as the assay employs protein A - coated fluomicrospheres (beads containing scintillant). The assay system was suitable for the quantitation of 0.03 to 2 pmol of 1-hexadecyl-2-acetyl-sn-glycero-3- phosphocholine. The cross-reactivity was high with 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine but was very low with PAF analogs such as 1-alkyl- and 1-acyl-2-lyso-sn-glycero-3-phosphocholine, 1-acyl-2-acetyl-sn-glycero-3-phosphocholine, and 1-alk-1'-enyl-2-acetyl-sn-glycero-3-phosphocholine. The specificity of SPRIA was higher than that of bioassay (platelet degranulation assay). PAF receptor antagonists (L-652,731, WEB2086, and FR900452) at up to 10 nmol per tube had no affect on the SPRIA. These observations indicate that the specificity of the PAF antibody is quite different from that of the platelet receptor. The values obtained using SPRIA for the measurement of PAF produced in polymorphonuclear leukocytes with stimuli are comparable to those obtained by SIM/GC/MS analysis.

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Citations

Jan 1, 1992·Life Sciences·A TokumuraH Tsukatani
Mar 1, 1994·Prostaglandins·D L KaminskiY G Deshpande
May 1, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·M KatoK Nakata
Mar 1, 1992·The Journal of Pharmacy and Pharmacology·A PintoF Capasso
Jul 2, 2003·Journal of Leukocyte Biology·Amanda J TonksSimon K Jackson
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S R ThomY Manevich
Jul 25, 1997·The Journal of Biological Chemistry·A J Ammit, C O'Neill
Nov 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J SugataniK Saito

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