Development of a novel system for isolating genes involved in predator-prey interactions using host independent derivatives of Bdellovibrio bacteriovorus 109J

BMC Microbiology
Adrian A MedinaDaniel Kadouri

Abstract

Bdellovibrio bacteriovorus is a gram-negative bacterium that preys upon other gram-negative bacteria. Although the life cycle of Bdellovibrio has been extensively investigated, very little is known about the mechanisms involved in predation. Host-Independent (HI) mutants of B. bacteriovorus were isolated from wild-type strain 109J. Predation assays confirmed that the selected HI mutants retained their ability to prey on host cells grown planktonically and in a biofilm. A mariner transposon library of B. bacteriovorus HI was constructed and HI mutants that were impaired in their ability to attack biofilms were isolated. Transposon insertion sites were determined using arbitrary polymerase chain reaction. Ten HI transposon mutants mapped to genes predicted to be involved in mechanisms previously implicated in predation (flagella, pili and chemotaxis) were further examined for their ability to reduce biofilms. In this study we describe a new method for isolating genes that are required for Bdellovibrio biofilm predation. Focusing on mechanisms that were previously attributed to be involved in predation, we demonstrate that motility systems are required for predation of bacterial biofilms. Furthermore, genes identified in this stud...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1995·International Journal of Food Microbiology·R Dewanti, A C Wong
Oct 1, 1993·PCR Methods and Applications·G Caetano-Anollés
Sep 1, 1996·Molecular Plant-microbe Interactions : MPMI·M SimonsB J Lugtenberg
Jun 1, 1996·Journal of Industrial Microbiology·M M DoolittleD E Caldwell
Dec 24, 1997·Trends in Microbiology·R J Palmer, D C White
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·B J AkerleyJ J Mekalanos
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·E J RubinJ J Mekalanos
Apr 27, 2000·Journal of Bacteriology·P Watnick, R Kolter
Aug 19, 2000·Infection and Immunity·N J GoldenD W Acheson
Aug 21, 2001·Archives of Microbiology·G Barel, Edouard Jurkevitch
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Jul 8, 2005·Applied and Environmental Microbiology·Daniel Kadouri, George A O'Toole
Aug 11, 2005·Molecular Microbiology·Lori L Burrows
Apr 20, 2006·Journal of Bacteriology·Hee Jung Lee, Kelly T Hughes
Nov 14, 2006·Applied and Environmental Microbiology·Daniel KadouriGeorge A O'Toole
Apr 10, 2007·Journal of Bacteriology·Katy J EvansR Elizabeth Sockett
Jun 15, 2007·Applied and Environmental Microbiology·Susan R Steyert, Silvia A Pineiro
Jan 1, 1995·Microbial Ecology·H N WilliamsW A Falkler

Citations

Jan 11, 2011·Molecular Oral Microbiology·Aliza Dashiff, Daniel Kadouri
Jul 16, 2008·Applied and Environmental Microbiology·John J TudorAdam Zwolak
Feb 1, 2011·Journal of Bacteriology·Nicole RoschanskiEckhard Strauch
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Carmen L GiltnerLori L Burrows
Jul 7, 2009·Annual Review of Microbiology·R Elizabeth Sockett
Jan 12, 2010·PloS One·Carey LambertR Elizabeth Sockett
Jul 11, 2009·The Open Microbiology Journal·Aliza Dashiff, Daniel Kadouri
May 30, 2009·Plasmid·Robert M Q ShanksGeorge A O'Toole
Feb 19, 2009·Research in Microbiology·Adrian A Medina, Daniel Kadouri
Nov 20, 2014·PloS One·Ryan M Chanyi, Susan F Koval
Dec 30, 2016·Environmental Microbiology Reports·Jess Tyson, R Elizabeth Sockett
Dec 30, 2015·Applied and Environmental Microbiology·Somdatta MukherjeeDaniel Kadouri
May 1, 2020·Frontiers in Microbiology·Eleni BratanisEwa Bukowska-Faniband
Oct 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Romain MercierTâm Mignot

Methods Mentioned

BETA
PCR

Related Concepts

Bdellovibrio
Chemotaxis
DNA Transposons
DNA, Bacterial
Flagella
Genes, Bacterial
Locomotion
Bacterial Pilus
Streak Plate Count
Insertion Mutation

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