Development of a novel three-dimensional in vitro model of oral Candida infection

Microbial Pathogenesis
A Dongari-Bagtzoglou, H Kashleva

Abstract

Oropharyngeal candidiasis is emerging as a serious health problem in the elderly as well as other chronically immunosuppressed patient populations. Several approaches have been used to study the interactions between Candida and the oral mucosa in vitro. Recently, three-dimensional organotypic systems of the oral mucosa have been developed, which provide an organizational complexity that is between the culture of single cell types and organ cultures in vivo. In this report we describe the development of a novel three-dimensional system of the human oral mucosa based on an immortalized oral keratinocyte cell line. Unlike the commercially available cell line systems, this system also contains a connective tissue cell component, which ensures the quality and resemblance of the tissue model to the human oral masticatory mucosa and submucosa. Using a panel of Candida albicans strains with variable virulence in vivo, we showed that the extent of tissue damage, fungal invasion and host inflammatory response in this system was proportional to the well-documented in vivo virulence potential of these strains. Therefore, this investigation has added another useful tool in the study of host pathogen interactions in oral candidiasis.

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Citations

Nov 18, 2008·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·M SchallerB Hube
May 10, 2007·Nature Protocols·Anna Dongari-Bagtzoglou, Helena Kashleva
Apr 5, 2007·Nature Protocols·Martin SchallerBernhard Hube
May 1, 2008·PloS One·Peter SouthernDana A Davis
Nov 19, 2010·PLoS Pathogens·Jianing N SunScott G Filler
May 11, 2010·Journal of Periodontology·Wen Lin ChaiRichard van Noort
Dec 17, 2008·Future Microbiology·Rosalía Diez-Orejas, Elena Fernández-Arenas
Jun 12, 2014·Applied Microbiology and Biotechnology·Tine De RyckBarbara Vanhoecke
Nov 10, 2013·Disease Models & Mechanisms·Venkata Ramanarao ParasaMaria Lerm
Apr 3, 2008·Expert Review of Anti-infective Therapy·Anna Dongari-Bagtzoglou
Feb 8, 2011·Microbial Pathogenesis·Nishant P YadevMartin H Thornhill
Mar 27, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mitchell KlausnerJoseph Kubilus
Apr 22, 2010·Mycoses·J A M S Jayatilake, L P Samaranayake
Jul 12, 2011·Current Opinion in Microbiology·Shantanu Ganguly, Aaron P Mitchell
Nov 15, 2013·Virulence·Edina K Szabo, Donna M Maccallum
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Feb 21, 2018·Journal of Medical Microbiology·Daniel J MorseDavid W Williams
Mar 7, 2020·Tissue Engineering. Part B, Reviews·Fahimeh TabatabaeiLobat Tayebi
Jan 10, 2018·Molecular Oral Microbiology·T SobueA Dongari-Bagtzoglou
Feb 12, 2020·The ISME Journal·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
Feb 2, 2021·FEMS Microbiology Reviews·Antonia LastBernhard Hube
Nov 5, 2019·Bio-protocol·Takanori SobueAnna Dongari-Bagtzoglou
Nov 4, 2019··Martinna BertoliniAnna Dongari-Bagtzoglou

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