Development of a Novel, Ultra-rapid Biosensor for the Qualitative Detection of Hepatitis B Virus-associated Antigens and Anti-HBV, Based on "Membrane-engineered" Fibroblast Cells with Virus-Specific Antibodies and Antigens.

Sensors
Antonios PerdikarisSpiridon E Kintzios

Abstract

A novel miniature cell biosensor detection system for the detection of Hepatis B virus (HBV)-associated antigens and anti-HBV is described. The biosensor is based on "membrane-engineered" Vero fibroblast cells immobilized in an alginate matrix. The membrane-engineering process involved the electroinsertion of anti-HBV specific antibodies (anti-HBs, anti-HBe) or antigens (HBsAg) in the membranes of the Vero cells. The attachment of a homologous antigen to the electroinserted antibody (or, respectively, of the antibody to the electroinserted antigen) triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the Bioelectric Recognition Assay (BERA). The sensor was used for screening 133 clinical blood serum samples according to a double-blind protocol. Considerably higher sensor responses were observed against HBV-positive samples, compared with responses against negative samples or samples positive for heterologous hepatitis viruses such as Hepatitis C (HCV) virus. Detection of anti-HBs antibodies was made possible by using a biosensor based on immobilized Vero cells bearing the respective antigen (HBsAg). The observed response was rapid (45 sec) and...Continue Reading

References

Jun 8, 2001·Biosensors & Bioelectronics·S KintziosR Levin
Oct 30, 2001·Journal of Pharmaceutical and Biomedical Analysis·Xiaodong ZhouJiming Hu
Nov 15, 2003·Biosensors & Bioelectronics·Rebecca J Whelan, Richard N Zare
Apr 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Paul TaylorMark Atkins
Aug 11, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yingxue ZhangFeng Yang
Oct 24, 2007·Biosensors & Bioelectronics·Chunyan YaoWeiling Fu
Nov 6, 2007·Mini Reviews in Medicinal Chemistry·Spiridon E Kintzios
Nov 4, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Suw Young Ly, Nam Sun Cho

❮ Previous
Next ❯

Citations

Apr 13, 2011·Talanta·Vassileios VarelasSpiridon Kintzios
Jun 9, 2012·Journal of Biomedical Materials Research. Part a·Swati MishraPaul Calvert
Jul 26, 2011·Journal of Virological Methods·Antonios PerdikarisSpiridon Kintzios
Jan 1, 2009·Sensors·Joseph Irudayaraj
Oct 31, 2014·World Journal of Gastroenterology : WJG·Sibnarayan DattaVijay Veer

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
surface plasmon resonance
biosensor
Assay

Software Mentioned

Doc
InstaCal
It® LS Image

Related Concepts

Related Feeds

Bartonellosis

Bartonellosis is an infection caused by bacteria in the genus Bartonella and can present as a variety of diseases depending on the organism involved. While asymptomatic infection has been reported, immunocompromised individuals are more susceptible to severe infection. Find the latest research on bartonellosis here.