Development of a novel ultrasensitive enzyme immunoassay for human glutamic acid decarboxylase 65 antibody

Annals of Clinical Biochemistry
Satoshi NumataSeiichi Hashida

Abstract

We developed a novel, ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for determination of glutamic acid decarboxylase autoantibody concentrations in serum samples from patients with type 2 diabetes. We developed an immune complex transfer enzyme immunoassay for glutamic acid decarboxylase autoantibody and measured glutamic acid decarboxylase autoantibody from 22 patients with type 1 diabetes, 29 patients with type 2 diabetes, and 32 healthy controls. A conventional ELISA kit identified 10 patients with type 1 diabetes and one patient with type 2 diabetes as glutamic acid decarboxylase autoantibody positive, whereas 15 patients with type 1 diabetes and six patients with type 2 diabetes were identified as glutamic acid decarboxylase autoantibody positive using immune complex transfer enzyme immunoassay. Immune complex transfer enzyme immunoassay is a highly sensitive and specific assay for glutamic acid decarboxylase autoantibody and might be clinically useful for diabetic onset prediction and early diagnosis.

Citations

May 1, 1993·Diabetes Care·T KobayashiK Kosaka
Jan 1, 1995·Biotechnology Annual Review·S HashidaE Ishikawa
Mar 3, 1999·Autoimmunity·R Juneja, J P Palmer
May 22, 2002·Annals of the New York Academy of Sciences·Tetsuro KobayashiJunichi Yokoyama
Apr 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Helen BrookingBernard Rees Smith
Apr 10, 2008·The Journal of Clinical Endocrinology and Metabolism·Taro MaruyamaTetsuro Kobayashi
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Ramachandra G NaikJerry P Palmer
Nov 12, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Eiji KawasakiKatsumi Eguchi
Jun 7, 2012·Clinical Biochemistry·Satoshi NumataSeiichi Hashida

Related Concepts

Diabetes Mellitus, Non-Insulin-Dependent
Maleimides
Autoantibodies
Galactosidase Activity
Cirrhosis, Familial
Determination Aspects
Glutamate Decarboxylase
Sulfhydryl Compounds
Phosphoglycerate Dehydrogenase Activity
Lysine

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