PMID: 9174636Jan 1, 1997Paper

Development of a pan-retrovirus detection system for multiple sclerosis studies

Acta Neurologica Scandinavica. Supplementum
P W TukeJ A Garson

Abstract

Although recent claims implicating HTLV-1 in multiple sclerosis (MS) have been refuted, several reports suggest that another, hitherto uncharacterised, retrovirus may be involved. We have developed and applied a novel PCR-based strategy to explore this possibility. Degenerate oligonucleotides were used in a semi-nested format to amplify, from reverse-transcribed RNA, a region of the pol gene which is well conserved amongst all known retroviruses. The 'pan-retrovirus' detection system was shown to be capable of detecting diverse retroviruses including human lentivirus, human oncovirus, simian D-type virus and murine oncovirus. The 'pan-retrovirus' technique identified a novel retroviral sequence, designated MSRV-cpol, in the serum of an MS patient and also in purified virions from MS patient-derived tissue cultures. Sequence comparisons suggest that in the pol gene MSRV is related (approximately 75% homology) to the endogenous retroviral element ERV9. These findings lend further support to the concept of retroviral involvement in MS.

References

Dec 1, 1992·Annals of Medicine·Y A KesäniemiA Rantala
Jan 1, 1992·British Medical Bulletin·P G Burney
Apr 6, 1991·Lancet·H PerronJ M Seigneurin
Oct 1, 1991·Journal of Neurology, Neurosurgery, and Psychiatry·P Rudge
Mar 1, 1991·Neurology·G D EhrlichS J Greenberg
May 1, 1991·Journal of Neuroimmunology·J SatohL F Kastrukoff
Jan 4, 1990·Nature·G Sarkar, S S Sommer
Jul 26, 1990·Nature·B FurrerJ Lüthy
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D H Mack, J J Sninsky
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S J GreenbergB J Poiesz
Mar 1, 1989·The Quarterly Review of Biology·R F DoolittleM A McClure
May 1, 1989·Journal of Cellular Biochemistry·M Hatanaka, S H Nam
May 18, 1989·Nature·S Kwok, R Higuchi
Feb 16, 1989·Nature·B H Waksman
Jan 1, 1988·Annals of Neurology·M B Gardner
Jan 1, 1985·British Medical Bulletin·C A Mims
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·P B ChallonerM Chang
Nov 1, 1994·The Journal of Experimental Medicine·K BankiA Perl
Jul 1, 1993·Trends in Microbiology·A Perl, K Banki
Oct 1, 1993·Clinical Microbiology Reviews·J F Kurtzke
Jul 1, 1957·Journal of Neuropathology and Experimental Neurology·B SIGURDSSONH GRIMSSON

❮ Previous
Next ❯

Citations

Sep 3, 2003·Journal of Virological Methods·Wolfgang SeifarthChristine Leib-Mösch
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·H KarlssonR H Yolken
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·H PerronB Mandrand
Apr 30, 1999·AIDS Research and Human Retroviruses·P OlssonJ Blomberg
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Cécile VoissetDavid J Griffiths
Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Fang Li, Håkan Karlsson
May 19, 2009·Journal of the Neurological Sciences·Hervé PerronJacques Portoukalian
Jul 21, 2005·The British Journal of Dermatology·J-P MolèsJ-J Guilhou
Apr 25, 2007·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Steven O A OluwoleHåkan Karlsson
Mar 27, 2003·Neuroreport·Håkan Karlsson
Jul 18, 2000·Journal of Medical Virology·W D AndrewsJ A Garson
Jan 1, 1997·Acta Neurologica Scandinavica. Supplementum·H PerronB Mandrand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.