Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus

Analytical and Bioanalytical Chemistry
Taek Jin BaekGi Hun Seong

Abstract

We describe a DNA microarray system using a bipolar integrated circuit photodiode array (PDA) chip as a new platform for DNA analysis. The PDA chip comprises an 8 x 6 array of photodiodes each with a diameter of 600 microm. Each photodiode element acts both as a support for an immobilizing probe DNA and as a two-dimensional photodetector. The usefulness of the PDA microarray platform is demonstrated by the detection of high-risk subtypes of human papilloma virus (HPV). The polymerase chain reaction (PCR)-amplified biotinylated HPV target DNA was hybridized with the immobilized probe DNA on the photodiode surface, and the chip was incubated in an anti-biotin antibody-conjugated gold nanoparticle solution. The silver enhancement by the gold nanoparticles bound to the biotin of the HPV target DNA precipitates silver metal particles at the chip surfaces, which block light irradiated from above. The resulting drop in output voltage depends on the amount of target DNA present in the sample solution, which allows the specific detection and the quantitative analysis of the complementary target DNA. The PDA chip showed high relative signal ratios of HPV probe DNA hybridized with complementary target DNA, indicating an excellent capabili...Continue Reading

References

Jul 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C S HolgateC C Bird
Apr 4, 1998·Acta Anaesthesiologica Scandinavica·Z J Koscielniak-NielsenL Hesselbjerg
Jan 23, 1999·Nature Genetics·P O Brown, D Botstein
Sep 8, 2000·Science·T A TatonR L Letsinger
Dec 2, 2000·Analytical Chemistry·J A FergusonD R Walt
Jul 31, 2001·Analytical Biochemistry·I AlexandreJ Remacle
Oct 27, 2001·Fresenius' Journal of Analytical Chemistry·L R AllainT Vo-Dinh
Jul 16, 2002·Annual Review of Biomedical Engineering·Michael J Heller
Jan 8, 2003·International Journal of Cancer. Journal International Du Cancer·Ethel-Michele de Villiers
Jan 30, 2003·Analytical Chemistry·Dimitra N Stratis-CullumTuan Vo-Dinh
Mar 21, 2003·Journal of Proteome Research·Cheng C WangJocelyn Burke
Aug 30, 2003·Analytical Chemistry·James P Landers
Dec 29, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Mary Manning, Gareth Redmond
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
May 26, 2005·Journal of the American Chemical Society·Hui DuTodd D Krauss
Jul 12, 2005·Biomacromolecules·Thomas AmeringerMartin Moeller
Mar 16, 2006·Analytical Chemistry·Robin Hui LiuAndy McShea
Aug 8, 2006·Biosensors & Bioelectronics·Joon Myong SongHo Taik Kwan
Aug 31, 2006·Methods in Enzymology·Jerilyn A Timlin
Sep 15, 2006·Analytical Chemistry·Jiangwei LiEdward S Yeung
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jingyue JuNicholas J Turro
Feb 1, 2007·Analytical Chemistry·Jose M Moran-MirabalDavid M Lin

❮ Previous
Next ❯

Citations

Aug 12, 2009·Journal of Virological Methods·Thomas BrandstetterJürgen Rühe
Mar 11, 2015·Reviews in Medical Virology·Jiezhong ChenZhi Ping Xu
Apr 8, 2010·Expert Review of Molecular Diagnostics·Seung Min Yoo, Sang Yup Lee
Dec 2, 2011·Chemical Society Reviews·Lev Dykman, Nikolai Khlebtsov
Nov 3, 2010·Analytica Chimica Acta·Rebecca L CaygillPaul A Millner
Nov 3, 2021·Nano Convergence·Jessica A Kemp, Young Jik Kwon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.