Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis

PLoS Neglected Tropical Diseases
Benoît StijlemansStefan Magez

Abstract

Extracellular trypanosomes can cause a wide range of diseases and pathological complications in a broad range of mammalian hosts. One common feature of trypanosomosis is the occurrence of anemia, caused by an imbalance between erythropoiesis and red blood cell clearance of aging erythrocytes. In murine models for T. brucei trypanosomosis, anemia is marked by a very sudden non-hemolytic loss of RBCs during the first-peak parasitemia control, followed by a short recovery phase and the subsequent gradual occurrence of an ever-increasing level of anemia. Using a newly developed quantitative pHrodo based in vitro erythrophagocytosis assay, combined with FACS-based ex vivo and in vivo results, we show that activated liver monocytic cells and neutrophils as well as activated splenic macrophages are the main cells involved in the occurrence of the early-stage acute anemia. In addition, we show that trypanosomosis itself leads to a rapid alteration of RBC membrane stability, priming the cells for accelerated phagocytosis.

References

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Citations

Mar 24, 2016·Trends in Parasitology·Monica R MugnierDanae Schulz
Sep 16, 2016·PLoS Pathogens·Benoît StijlemansPatrick De Baetselier
Jun 17, 2015·Parasite Immunology·J CnopsS Magez
Feb 18, 2017·Frontiers in Immunology·Thomas R L KleiRobin van Bruggen
Sep 25, 2017·Frontiers in Immunology·Hui WuMeiqing Shi
Jul 27, 2018·Scientific Reports·Guy CaljonJan Van Den Abbeele
Apr 19, 2018·Essays in Biochemistry·Michael J Cipriano, Stephen L Hajduk
Jan 11, 2020·Haematologica·João V NevesPedro N S Rodrigues
Feb 19, 2021·Molecular Immunology·Izadora Volpato RossiMarcel Ivan Ramirez
Aug 28, 2021·International Journal of Molecular Sciences·Christina MertensMichaela Jung
Sep 16, 2021·PLoS Pathogens·Henrique MachadoLuisa M Figueiredo

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Methods Mentioned

BETA
FACS
flow cytometry
FCS
fluorescence microscopy
PECs
ELISA

Software Mentioned

FlowJo
GraphPad Prism
GraphPad

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