Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus

Avian Diseases
Chiou-Lin ChenPoa-Chun Chang

Abstract

This article reports the complete nucleotide sequences of four duck circovirus (DuCV) isolates from sick ducks in Taiwan and development of a polymerase chain reaction (PCR) for detection and differentiation of goose circovirus (GoCV) and DuCV. Sequence comparison showed that Taiwanese DuCV isolates had 82.5%-83.8% nucleotide sequence identity to the German and North American DuCV isolates. This is the first report on the presence of DuCV and its associated diseases outside Germany. A PCR test was developed using a universal primer pair based on conserved sequences present in the genomes of GoCV and DuCV. This PCR test could detect and differentiate between GoCV and DuCV by the size of PCR product each virus produced (256 bp for GoCV and 228 bp for DuCV). Application of this PCR test to samples of bursa of Fabricius from sick birds in the field showed that 9 of 26 goose samples contained GoCV, while 13 of 34 duck samples contained DuCV. This PCR test could serve as a fast and sensitive method for detection and differentiation of DuCV and GoCV.

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Citations

Jun 12, 2012·Virologica Sinica·Xing-xiao ZhangShi-jin Jiang
May 4, 2013·Veterinary Microbiology·Se-Yeoun ChaHyung-Kwan Jang
Feb 14, 2013·Genome Announcements·Zhilong ZhangAnchun Cheng
Jun 15, 2012·Virology Journal·Grzegorz WoźniakowskiElżbieta Samorek-Salamonowicz
Dec 25, 2013·The Journal of Veterinary Medical Science·Se-Yeoun ChaHyung-Kwan Jang
Apr 28, 2012·Veterinary Microbiology·Qi-Wang XiangShi-Jin Jiang
Aug 17, 2010·Veterinary Microbiology·Dan WangDabing Zhang
Apr 23, 2010·Veterinary Microbiology·Shao-Ning LiuShi-Jin Jiang
May 31, 2015·Virology Journal·Pengfei LiAnchun Cheng
Jan 28, 2016·Avian Pathology : Journal of the W.V.P.A·Ning LiuDabing Zhang
Sep 30, 2014·Veterinary Microbiology·Zhiguo LiShijin Jiang
Oct 29, 2014·Archives of Virology·Zhicheng ZhangDingzhen Dai
Nov 1, 2016·Genome Announcements·Wenchao SunNingyi Jin
Apr 28, 2007·Avian Diseases·Alejandro BandaKarel A Schat
Jun 13, 2020·Transboundary and Emerging Diseases·Jun JiQingmei Xie
Jul 29, 2011·Avian Diseases·Guanghua FuJiansheng Lin
Oct 7, 2020·Avian Pathology : Journal of the W.V.P.A·Yang-Chang TuYen-Ping Chen

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Datasets Mentioned

BETA
DQ10076
AY394721
DQ166836-166838

Methods Mentioned

BETA
PCR
electron microscopy
electrophoresis

Software Mentioned

NEIGHBOR
PHYLIP
GeneQuest
LASERGENE
MegAlign
BLAST
Basic Local Alignment Search Tool ( BLAST )
CONSENSE
Clustal W
Seqman

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