Development of a polyvalent assay system for lead identification

Bioorganic & Medicinal Chemistry Letters
Frank LoveringKristie Bridges

Abstract

In an effort to identify new approaches to lead discovery a polyvalent assay was developed to allow identification of weak inhibitors. This approach involves the polyvalent display of a protein binder off a Tenta-gel scaffold and the generation of a polyvalent display of protein by biotinylation followed by complexation with fluorescently labeled streptavidin. Subsequent exposure of the streptavidin complexed protein to Tenta-gel beads with active protein binders results in fluorescent beads, which are easily viewed under a fluorescent microscope.

References

Jul 8, 1999·Journal of Medicinal Chemistry·P J HajdukS W Fesik
Jun 8, 2000·Bioorganic & Medicinal Chemistry Letters·M RabinowitzG Terstappen
Dec 5, 2000·Current Opinion in Chemical Biology·L L KiesslingL E Strong
Mar 29, 2001·Journal of Pharmacological and Toxicological Methods·C A Lipinski
Jun 21, 2001·Journal of Chemical Information and Computer Sciences·M M HannG Harper
Dec 12, 2001·Bioorganic & Medicinal Chemistry·Myung-Gi Baek, René Roy
Dec 26, 2001·Journal of the American Chemical Society·J M GarganoW J Lees
Jan 5, 2002·Organic Letters·Eric K Woller, Mary J Cloninger
Apr 1, 1997·Chemical Reviews·Kit S. LamViktor Krchnák
Feb 21, 2002·Journal of the American Chemical Society·Christopher W CairoLaura L Kiessling
May 31, 2002·Journal of Medicinal Chemistry·Daniel F VeberKenneth D Kopple
Feb 5, 2003·Drug Discovery Today·Gilbert M Rishton

❮ Previous
Next ❯

Citations

Jan 29, 2009·Academic Medicine : Journal of the Association of American Medical Colleges·Elliot Wakeam
Aug 27, 2009·Academic Medicine : Journal of the Association of American Medical Colleges·Jerome P Kassirer
Oct 24, 2009·Current Opinion in Critical Care·Julian Bion
Jun 4, 2010·Academic Medicine : Journal of the Association of American Medical Colleges·Frederick S Sierles
Sep 21, 2010·Academic Medicine : Journal of the Association of American Medical Colleges·Darren E ZinnerEric G Campbell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.