Development of a portable and disposable NS1 based electrochemical immunosensor for early diagnosis of dengue virus

Analytica Chimica Acta
Mian Hasnain NawazJean Louis Marty

Abstract

The present study represents fabrication of nonstructural antibody (NS1) based immunosensor coupled with bovine serum albumin (BSA) modified screen printed carbon electrodes (SPCE) as transducing substrate for the early diagnosis of dengue virus. The anti-NS1 monoclonal antibody was immobilized on electro grafted BSA surface of working electrode. The electrons transfer resistance before and after NS1 attachment was monitored as a function of its concentration to perform the qualitative and quantitative analysis. The as prepared impedimetric immunosensor successfully detected the dengue virus protein with enhanced limit of detection (0.3 ng/mL) and linear range (1-200 ng/mL). The selectivity of the designed device was further elaborated with several interfering analytes and was finally demonstrated with human serum samples. The extravagant selectivity, sensitivity and easier fabrication protocol corroborate the potential applications of such immunosensor for practical diagnosis of dengue virus.

Citations

Feb 27, 2020·Applied Biochemistry and Biotechnology·Rabia ArshadUsman Latif
Mar 11, 2020·Frontiers in Bioengineering and Biotechnology·Estefânia Vangelie Ramos CamposLeonardo Fernandes Fraceto
Jun 14, 2020·International Journal of Antimicrobial Agents·Atul SharmaJean Louis Marty
Jan 19, 2021·Journal of Electroanalytical Chemistry·Hüseyin Oğuzhan KayaSeda Nur Topkaya
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Katarína Nemčeková, Ján Labuda
Feb 2, 2021·Acta Tropica·Jonatas Lobato DuarteMarlus Chorilli
Mar 12, 2021·Biosensors & Bioelectronics·K Yugender GoudK Vengatajalabathy Gobi
Apr 20, 2021·Analytica Chimica Acta·Laís Canniatti BrazacaEmanuel Carrilho
Jun 22, 2021·Analytical and Bioanalytical Chemistry·Priscila D MendonçaRosa F Dutra
Jun 29, 2021·Trends in Analytical Chemistry : TRAC·Mazhar SherStefano Cinti
Jul 20, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Shipra SolankiGajjala Sumana
Aug 9, 2021·Talanta·Taís Aparecida Reis CordeiroLucas Franco Ferreira

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