Development of a Practical Method to Detect Noroviruses Contamination in Composite Meals

Food and Environmental Virology
Hiroyuki SaitoMamoru Noda

Abstract

Various methods to detect foodborne viruses including norovirus (NoV) in contaminated food have been developed. However, a practical method suitable for routine examination that can be applied for the detection of NoVs in oily, fatty, or emulsive food has not been established. In this study, we developed a new extraction and concentration method for detecting NoVs in contaminated composite meals. We spiked NoV-GI.4 or -GII.4 stool suspension into potato salad and stir-fried noodles. The food samples were suspended in homogenizing buffer and centrifuged to obtain a food emulsion. Then, anti-NoV-GI.4 or anti-NoV-GII.4 rabbit serum raised against recombinant virus-like particles or commercially available human gamma globulin and Staphylococcus aureus fixed with formalin as a source of protein A were added to the food emulsion. NoV-IgG-protein A-containing bacterial complexes were collected by centrifugation, and viral RNA was extracted. The detection limits of NoV RNA were 10-35 copies/g food for spiked NoVs in potato salad and stir-fried noodles. Human gamma globulin could also concentrate other NoV genotypes as well as other foodborne viruses, including sapovirus, hepatitis A virus, and adenovirus. This newly developed method ca...Continue Reading

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Citations

Sep 30, 2018·Transboundary and Emerging Diseases·Wataru YamazakiKenji Tsukamoto
Jul 28, 2016·Current Opinion in Infectious Diseases·Miren Iturriza-Gomara, Sarah J O'Brien
Apr 18, 2018·Emerging Infectious Diseases·Naomi SakonHirokazu Kimura
Nov 15, 2020·International Journal of Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Oct 22, 2019·International Journal of Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
May 4, 2021·International Journal of Food Microbiology·Jade DerrickMiren Iturriza-Gómara
Oct 4, 2021·Risk Analysis : an Official Publication of the Society for Risk Analysis·Kriangsak RuchusatsawatSuphachai Nuanualsuwan

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