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Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in Gram-negative soil bacteria

Journal of Microbiological Methods

Oct 1, 2012

Esther EnowashuMarion Engel

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Abstract

A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA fro...read more

Mentioned in this Paper

DNA Sequence
Sampling - Surgical Action
Hyphomicrobium denitrificans
Homologous Gene
Oligonucleotide Primers
Sequence Analysis
Thiobacillus denitrificans
Soil Microbiology
Biologic Degeneration
Bordetella petrii
3
16
Paper Details
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  • Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in Gram-negative soil bacteria

    Journal of Microbiological Methods

    Oct 1, 2012

    Esther EnowashuMarion Engel

    PMID: 22796679

    DOI: 10.1016/j.mimet.2012.07.001

    Abstract

    A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA fro...read more

    Mentioned in this Paper

    DNA Sequence
    Sampling - Surgical Action
    Hyphomicrobium denitrificans
    Homologous Gene
    Oligonucleotide Primers
    3
    16

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    • References15
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    • References15
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