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Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in Gram-negative soil bacteria

Journal of Microbiological Methods

Jul 17, 2012

Esther EnowashuMarion Engel

Abstract

A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA fro...read more

Mentioned in this Paper

Gram-Positive Bacteria
Abnormal Degeneration
Dimethyl Sulfoxide
Agrobacterium tumefaciens
Bordetella petrii
Amino Acids, I.V. solution additive
Pseudomonas putida
Peptide Hydrolases
Ncbi Taxonomy
Aminooligopeptidase
3
16
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12
  • References15
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Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in Gram-negative soil bacteria

Journal of Microbiological Methods

Jul 17, 2012

Esther EnowashuMarion Engel

PMID: 22796679

DOI: 10.1016/j.mimet.2012.07.001

Abstract

A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA fro...read more

Mentioned in this Paper

Gram-Positive Bacteria
Abnormal Degeneration
Dimethyl Sulfoxide
Agrobacterium tumefaciens
Bordetella petrii
Amino Acids, I.V. solution additive
Pseudomonas putida
Peptide Hydrolases
Ncbi Taxonomy
Aminooligopeptidase
3
16

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Oxygenation & Hydroxylation

Oxygenation is a chemical production that introduces oxygen atom into an organic compound. Hydroxylation is a chemical process that introduces a hydroxyl group (-OH) into an organic compound. In biochemistry, hydroxylation reactions are often facilitated by enzymes called hydroxylases. Cytochrome P450 (CYP) enzymes are expressed mainly in the liver and are active in mono-oxygenation and hydroxylation of various xenobiotics, including drugs and alcohols, as well as that of endogenous compounds such as steroids, bile acids, prostaglandins, leukotrienes and biogenic amines. Here is the latest research.

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Radiocarbon--a low-impact tool to study nutrient transport by soil fungi under field conditions

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Paper Details
References
  • References15
  • Citations
12
  • References15
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.
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