Development of a quantitative immunological assay for the study of spore coat synthesis and morphogenesis.

Journal of Bacteriology
D HornE S Golub

Abstract

A quantitative assay employing (125)I-labeled antibody has been developed for Bacillus cereus T spore coat protein. Populations of antibody molecules with various affinities for inner or outer coat can be prepared by selective adsorption to and elution from different coat preparations. Adsorption to and elution from intact spores results in an antibody preparation at least 15 times more reactive to outer coat. This antibody is useful for measuring the time and extent of spore coat maturation, i.e., outer spore coat formation. Rifampin inhibits the increase in content of this coat antigen.

References

Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D P Nierlich
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·W WehrliM Staehelin
Jan 1, 1968·Advances in Protein Chemistry·C Tanford
Apr 14, 1968·Journal of Molecular Biology·A I Aronson, P C Fitz-James
Mar 1, 1970·The Journal of Applied Bacteriology·P D Walker
Oct 1, 1971·Journal of Bacteriology·A I Aronson, P C Fitz-James
Mar 1, 1963·The Journal of Cell Biology·A D WARTHW G MURRELL
Dec 1, 1959·The Journal of Biophysical and Biochemical Cytology·I E Young, P C Fitz-James

Citations

Apr 1, 1981·Archives of Microbiology·W Wright, A I Aronson
Jan 1, 1987·Microbiology and Immunology·H I el-BelbasiM Kondo
Jun 1, 1976·Bacteriological Reviews·A I Aronson, P C Fitz-James
Mar 1, 1973·Journal of Bacteriology·H M ChengS C Holt
Jan 1, 1981·Journal of Bacteriology·A I Aronson
Mar 1, 1979·Journal of Bacteriology·N K Pandey, A I Aronson
Nov 1, 1987·Journal of Bacteriology·J RubikasV Stepanov
Jun 1, 1978·Journal of Bacteriology·G N StelmaP C Fitz-James

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.