Development of a rapid and sensitive europium (III) chelate microparticle-based lateral flow test strip for the detection and epidemiological surveillance of porcine epidemic diarrhea virus

Archives of Virology
Jianbo LiuLi Feng

Abstract

Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is the predominant cause of severe enteropathogenic diarrhea in swine. A simple, rapid, specific, and sensitive method is critical for monitoring PEDV on pig farms. In this study, a simple and rapid lateral flow immunoassay detection system that integrates europium (Eu) (III) chelate microparticles was developed to identify PEDV in fecal swabs. This newly developed diagnostic sandwich immunoassay utilizes lateral flow test strips (LFTSs). The fluorescence peak heights of the test line (HT) and the control line (HC) were measured using a fluorescence strip reader, and the HT/HC ratio was used for quantitation. The limit of detection of PEDV with this LFTS was ??ten times the median tissue culture infectious dose (TCID50) per mL??. Fecal swab samples were used to determine the cutoff value. Field samples, various PEDV strains and other viruses were used to determine the sensitivity and specificity of the Eu (III) chelate microparticle-based LFTSs, which were 97.8% and 100%, respectively, with a cutoff value of 0.05, as compared with reverse transcription polymerase chain reaction (RT-PCR). In samples from piglets experimentally infected with PEDV, the...Continue Reading

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Mar 21, 1998·Journal of Virological Methods·K IshikawaS Suzuki
Nov 29, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S Y KimB K Park
Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sunga ChoiSang Wook Oh
Aug 22, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gregory W StevensonKyoungjin J Yoon
Mar 23, 2019·Emerging Microbes & Infections·Dongming ZhaoZhigao Bu

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
electrophoresis
ELISA
sandwich immunoassay

Software Mentioned

GraphPad
GraphPad Prism
MedCalc

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