Abstract
A rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of Marek's disease virus (MDV) by amplification of conserved MDV meq gene sequences. LAMP is an innovative technique that allows the rapid detection of targeted nucleic acid sequences under isothermal conditions without the need for complex instrumentation. In this study, meq gene sequences were amplified successfully from different MDV strains by LAMP within 60min and no cross-reactivity was observed in a panel of related viruses that were associated with diseases of chickens. The detection limit of LAMP was 3.2 copies/million cells compared with 320 copies/million cells required for conventional PCR. Positive detection rates were assessed using either LAMP or PCR by examination of feather follicles that were collected from chickens infected experimentally with either strain J-1 (n=20) or strain Md5 (n=17), In addition to these samples, three isolates that were suspected to have been infected in the clinic were also tested. Results showed that the positive detection rate for LAMP was 95% (38/40), compared with 87.5% (35/40) and 90% (38/40) for strains J-1 and Md5 by PCR, respectively. These results...Continue Reading
References
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Sep 6, 2003·Journal of Clinical Microbiology·M EnosawaY Yokomizo
Jan 13, 2004·Journal of Clinical Microbiology·Manmohan ParidaKouichi Morita
May 28, 2004·Journal of Biochemical and Biophysical Methods·Yasuyoshi MoriTsugunori Notomi
Apr 9, 2005·Journal of Clinical Microbiology·Hang Minh PhamMisao Onuma
Oct 26, 2005·FEMS Microbiology Letters·Yukiko Hara-KudoMasanari Ikedo
Apr 25, 2006·Journal of Applied Microbiology·H-Y YehP H Klesius
Jun 21, 2008·Foodborne Pathogens and Disease·Feifei Han, Beilei Ge
Oct 18, 2008·Journal of Virological Methods·Qiang LiYong-Chang Cao
May 8, 2009·The Journal of Veterinary Medical Science·Oriel M M ThekisoeNoboru Inoue
Aug 1, 2009·Journal of Virological Methods·Jiangtao XuLimin Han
Jan 27, 2010·Journal of Virological Methods·Qing-mei XieYing-zuo Bi
Mar 23, 2010·Journal of Virological Methods·Xing-Juan ZhangHua-Ji Qiu
Apr 13, 2010·Journal of Virological Methods·Xue-Jun MaXiao-Ping Dong
May 4, 2010·Journal of Virological Methods·Xiaoyun DengXiaomei Wang
May 5, 2010·The American Journal of Tropical Medicine and Hygiene·Oriel M M ThekisoeNoboru Inoue
Citations
Nov 10, 2013·Pakistan Journal of Biological Sciences : PJBS·Shambhu Dayal SinghKuldeep Dhama
Feb 4, 2014·Virus Research·Xuemei LiuWeisheng Cao
Apr 27, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Hongchao LvChangjun Liu
Dec 22, 2017·Veterinary World·A J AdedejiT M Joannis