Development of a rapid assay methodology for antimicrobial susceptibility testing of Staphylococcus aureus

Diagnostic Microbiology and Infectious Disease
Janne O KoskinenAleksi E Soini

Abstract

Development of a new phenotypic technique for rapid antimicrobial susceptibility testing (AST) of methicillin-resistant Staphylococcus aureus is presented. The new technique combines bacterial culturing and specific immunometric detection in a single separation-free process. The technique uses dry chemistry reagents and the recently developed two-photon excitation detection technology, which allows online detection of bacterium-specific growth. The performance of the new technique was evaluated by monitoring the growth of S. aureus reference strains and determining their susceptibility to oxacillin. In the direct analysis of clinical specimens, method specificity and tolerance to interferences caused by other bacteria present in the sample are pivotal. Other bacteria can compete with the bacteria of interest for nutrients, for example. Specificity and tolerance were studied against Staphylococcus epidermidis reference strains. The results suggest that the new technique could allow rapid AST directly from clinical samples within 6 to 8 h. Such a rapid and simple testing methodology would be a valuable tool in clinical microbiology because it would shorten the turnaround times of microbiologic analyses. Advantages of the new appr...Continue Reading

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Citations

Apr 5, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T StenholmP Kotilainen
Mar 9, 2010·Journal of Virological Methods·Hanna SmolanderAleksi E Soini
Oct 5, 2013·Scandinavian Journal of Infectious Diseases·Teppo StenholmPirkko Kotilainen
Mar 21, 2012·International Journal of Environmental Health Research·Ana MaravićMaja Pavela-Vrančić
Oct 24, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Susan MeiringUNKNOWN GERMS-SA

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