Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method

Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Zhongyuan LiuDongyan Liu

Abstract

Blooms of the harmful jellyfish Cyanea nozakii, which are a severe nuisance to fisheries and tourisms, frequently occur in the northern East China Sea, Yellow Sea, and Bohai Sea. To provide early warning of this species, a simple and effective molecular method for identifying C. nozakii was developed using the loop-mediated isothermal amplification method (LAMP). The LAMP assay is highly specific and uses a set of four primers that target six different regions on the mitochondrial cytochrome c oxidase subunit I (COI) gene of C. nozakii. The amplification conditions, including the dNTP and betaine concentrations, the inner primer to outer primer concentration ratio, reaction time and temperature, were optimized. The LAMP assay amplified DNA extracted from tissue samples of C. nozakii but did not amplify DNA from other common scyphozoans and hydrozoans collected in the same region. In addition, the LAMP assay was more sensitive than conventional PCR. Therefore, the established LAMP assay is a sensitive, specific, fast, and easily performed method for detection of C. nozakii at different stages in their life cycle.

References

Oct 10, 1997·Nucleic Acids Research·W HenkeS A Loening
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
Jul 30, 2002·Molecular and Cellular Probes·K NagamineT Notomi
Jan 8, 2005·Science·Weiwei XianRuiyu Liu
Nov 11, 2008·Journal of Virological Methods·Chang-mu Chen, Shang-jin Cui
Jul 22, 2009·Toxicon : Official Journal of the International Society on Toxinology·Jinhua FengPengcheng Li
Jun 18, 2010·Marine Pollution Bulletin·Zhijun DongJohn K Keesing
Feb 19, 2011·Annual Review of Marine Science·Ann BucklinLeocadio Blanco-Bercial
Jun 15, 2012·Virology Journal·Grzegorz WoźniakowskiElżbieta Samorek-Salamonowicz
Aug 11, 2012·Advances in Marine Biology·Cathy H LucasChad Widmer

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